Sep 21, 2013

Overexpression of L1CAM is associated with tumor progression and prognosis via ERK signaling in gastric cancer

Annals of Surgical Oncology
Takeshi ItoYasuhiro Kodera

Abstract

L1 cell adhesion molecule (L1CAM), which belongs to the immunoglobulin superfamily, has recently been observed in a variety of human malignancies. However, its clinical implication in gastric cancer remains unclear. The aim of this study was to explore the role of L1CAM in gastric cancer and to analyze its correlation with tumor progression and prognosis. L1CAM expression was measured in human gastric cancer cell lines and knockdown was conducted using siRNA. Cell proliferation, invasion and migration ability was assessed in vitro. The downstream pathway of L1CAM was explored by western blot analysis. L1CAM expression was measured in 112 pairs of human gastric cancer and adjacent noncancerous tissues using real-time quantitative RT-PCR, and the correlation with clinicopathological features and prognosis was analyzed. L1CAM downregulation by siRNA significantly decreased cell proliferation, migration, and invasion in gastric cancer cell lines. Phosphorylated ERK levels began to decline more rapidly in L1CAM knockdown cells compared with parental cells. L1CAM overexpression was significantly correlated with local tumor cell growth (P = 0.041), distant metastasis (P = 0.047), and tumor stage (P = 0.031). The overall survival in pa...Continue Reading

  • References31
  • Citations12

Mentioned in this Paper

Biochemical Pathway
TNM Staging System
Apoptosis, Intrinsic Pathway
Immunoglobulin Activity
Malignant Neoplasm of Stomach
Stomach Carcinoma
RNA, Small Interfering
Cell Motility
Kinetic Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction

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