Overexpression of LOXIN Protects Endothelial Progenitor Cells From Apoptosis Induced by Oxidized Low Density Lipoprotein

Journal of Cardiovascular Pharmacology
Carlos VeasClaudio Aguayo

Abstract

Human endothelial progenitor cells (hEPC) are adult stem cells located in the bone marrow and peripheral blood. Studies have indicated that hEPC play an important role in the recovery and repair of injured endothelium, however, their quantity and functional capacity is reduced in several diseases including hypercholesterolemia. Recently, it has been demonstrated that hEPC express lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and its activation by oxidized low-density lipoprotein (ox-LDL) induces cellular dysfunction and apoptosis. This study aimed to investigate whether overexpression of LOXIN, a truncated isoform of LOX-1 that acts as a dominant negative, plays a protective role against ox-LDL-induced apoptosis in hEPC. Human endothelial progenitor cells exposed to ox-LDL showed a significant increase in LOX-1 expression, and apoptosis began at ox-LDL concentrations above 50 μg/mL. All hEPC apoptosed at 200 μg/mL ox-LDL. High LOXIN expression was generated using adenoviral systems in hEPC and SiHa cells transduced with 100 colony-forming units per cell. Transduced LOXIN localized to the plasma membrane and blocked ox-LDL uptake mediated by LOX-1. Overexpression of LOXIN protected hEPC from ox-LDL-induced apop...Continue Reading

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Citations

Feb 26, 2016·Microvascular Research·Valter Lubrano, Silvana Balzan
Feb 2, 2017·International Journal of Molecular Sciences·Barbara RizzacasaFrancesca Amati
Jul 23, 2016·International Journal of Molecular Sciences·Xiaojuan Ma, Yingmei Feng
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Feb 2, 2021·Heart & Lung : the Journal of Critical Care·Pouya SalehipourMohammad Hossein Modarressi
Feb 9, 2021·Frontiers in Cardiovascular Medicine·Torben MentrupBernd Schröder

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