Overexpression of MdbHLH104 gene enhances the tolerance to iron deficiency in apple

Plant Biotechnology Journal
Qiang ZhaoYu-Jin Hao

Abstract

Fe deficiency is a widespread nutritional disorder in plants. The basic helix-loop-helix (bHLH) transcription factors (TFs), especially Ib subgroup bHLH TFs which are involved in iron uptake, have been identified. In this study, an IVc subgroup bHLH TF MdbHLH104 was identified and characterized as a key component in the response to Fe deficiency in apple. The overexpression of the MdbHLH104 gene noticeably increased the H(+) -ATPase activity under iron limitation conditions and the tolerance to Fe deficiency in transgenic apple plants and calli. Further investigation showed that MdbHLH104 proteins bonded directly to the promoter of the MdAHA8 gene, thereby positively regulating its expression, the plasma membrane (PM) H(+) -ATPase activity and Fe uptake. Similarly, MdbHLH104 directly modulated the expression of three Fe-responsive bHLH genes, MdbHLH38, MdbHLH39 and MdPYE. In addition, MdbHLH104 interacted with 5 other IVc subgroup bHLH proteins to coregulate the expression of the MdAHA8 gene, the activity of PM H(+) -ATPase and the content of Fe in apple calli. Therefore, MdbHLH104 acts together with other apple bHLH TFs to regulate Fe uptake by modulating the expression of the MdAHA8 gene and the activity of PM H(+) -ATPase in...Continue Reading

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Citations

Aug 31, 2018·Plant & Cell Physiology·Feng-Pan WangYu-Jin Hao
Jun 25, 2019·Horticulture Research·Xian-Yan ZhaoYu-Jin Hao
May 24, 2020·Plant Biotechnology Journal·Qiang ZhaoYang-Yang Li
Jul 15, 2020·Plant Signaling & Behavior·Pengfei DiaoNana Ma
Mar 19, 2019·Frontiers in Plant Science·Guo-Qing SongVladimir Orbovic
Nov 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sun A KimMary Lou Guerinot
Apr 4, 2017·Journal of Experimental Botany·Gang LiangDiqiu Yu
Nov 28, 2020·Journal of Experimental Botany·Fei Gao, Christian Dubos
Nov 6, 2018·Free Radical Biology & Medicine·Takanori KobayashiNaoko K Nishizawa
Jun 22, 2020·Plant Science : an International Journal of Experimental Plant Biology·Jiu-Cheng ZhangYu-Jin Hao
Jan 19, 2021·Plant, Cell & Environment·Wei-Jian SunYu-Jin Hao
Apr 10, 2021·The Plant Journal : for Cell and Molecular Biology·Wenxing ChenZhengrong Luo
Oct 11, 2017·Molecular Plant·Christoph-Martin Geilfus

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Methods Mentioned

BETA
transgenic
immunoprecipitation PCR
electrophoretic mobility shift assay
two‐hybrid
immunoprecipitation

Software Mentioned

BlastX

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