Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Shi XiaoMee-Len Chye

Abstract

In Arabidopsis thaliana, a family of six genes encodes acyl-CoA-binding proteins (ACBPs) that show conservation at the acyl-CoA-binding domain. They are the membrane-associated ACBP1 and ACBP2, extracellularly targeted ACBP3, kelch-motif-containing ACBP4 and ACBP5, and 10-kDa ACBP6. The acyl-CoA domain in each of ACBP1 to ACBP6 binds long-chain acyl-CoA esters in vitro, suggestive of possible roles in plant lipid metabolism. We addressed here the use of Arabidopsis ACBPs in conferring lead [Pb(II)] tolerance in transgenic plants because the 10-kDa human ACBP has been identified as a molecular target for Pb(II) in vivo. We investigated the effect of Pb(II) stress on the expression of genes encoding Arabidopsis ACBP1, ACBP2 and ACBP6. We showed that the expression of ACBP1 and ACBP2, but not ACBP6, in root is induced by Pb(II) nitrate treatment. In vitro Pb(II)-binding assays indicated that ACBP1 binds Pb(II) comparatively better, and ACBP1 was therefore selected for further investigations. When grown on Pb(II)-containing medium, transgenic Arabidopsis lines overexpressing ACBP1 were more tolerant to Pb(II)-induced stress than the wild type. Accumulation of Pb(II) in shoots of the ACBP1-overepxressing plants was significantly hig...Continue Reading

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Citations

Nov 1, 2011·Tree Physiology·Daniela Di BaccioLuca Sebastiani
Mar 6, 2012·BMC Genomics·Jamshaid AliAkash Ranjan
Jul 14, 2010·Cellular and Molecular Life Sciences : CMLS·Aude MigeonMichel Chalot
Jan 23, 2016·Frontiers in Plant Science·Florence AuguyPatrick Doumas
Mar 5, 2016·Plant Physiology and Biochemistry : PPB·Jose A Aznar-MorenoJoaquín J Salas
Feb 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Steven PastorJay Shockey
Jan 10, 2016·Biochimica Et Biophysica Acta·Shiu-Cheung Lung, Mee-Len Chye
Dec 27, 2011·Plant Science : an International Journal of Experimental Plant Biology·Riza-Arief PutrantoPascal Montoro
Dec 15, 2015·Lipids·Zi-Wei Ye, Mee-Len Chye
Oct 12, 2010·New Biotechnology·Olga P Yurchenko, Randall J Weselake
Jan 6, 2009·Plant Physiology and Biochemistry : PPB·Shi Xiao, Mee-Len Chye
Jan 21, 2009·Plant Physiology and Biochemistry : PPB·Shuqing CaoXinliang Ma
Jun 24, 2010·The New Phytologist·Johnathan A Napier, Richard P Haslam
Sep 6, 2015·Protoplasma·Shiu-Cheung Lung, Mee-Len Chye
Apr 23, 2015·Progress in Lipid Research·Ditte NeessNils J Færgeman
Aug 26, 2009·Plant Signaling & Behavior·Qin-Fang ChenMee-Len Chye
Dec 17, 2009·Plant Signaling & Behavior·Shi XiaoMee-Len Chye
Nov 15, 2014·Plant & Cell Physiology·An-Shan HsiaoMee-Len Chye
Sep 30, 2014·PloS One·An-Shan HsiaoMee-Len Chye
Jun 24, 2016·Journal of Experimental Botany·Tingting FanShuqing Cao
Feb 9, 2017·Plant Science : an International Journal of Experimental Plant Biology·Li JiangHaimei Cao
May 5, 2017·Acta Crystallographica. Section D, Structural Biology·Ze Hua GuoMee Len Chye
Feb 10, 2018·Frontiers in Plant Science·Tai-Hua HuMee-Len Chye

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