Overexpression of metallothionein in CHO cells and its effect on cell killing by ionizing radiation and alkylating agents.

Carcinogenesis
H Lohrer, T Robson

Abstract

Metallothionein protein protects cells from the toxic effects of heavy metal ions. To establish its protective function against ionizing radiation and alkylating agents, a model system was created by transfecting two CHO cell lines (wild-type, K1-2 and X-ray sensitive, xrs-2 subclone Bc11) with the human metallothionein II-A (hMTII-A) gene integrated in a bovine papilloma derived autonomously replicating vector. The isolated transfectants are cadmium-resistant (Cdr), due to the overexpression of the hMTII-A gene. Their steady-state level of hMTII-A mRNA can be increased up to 40-fold after Cd treatment and 20-fold after induction with ionizing radiation. The transfected cell lines proved to be as sensitive as the recipient cell lines to ionizing radiation and bleomycin but the transfectants were significantly more resistant to N-methyl-N'-nitro-N'-nitrosoguanidine (MNNG) and mitomycin C (MMC). These results lead to the conclusion that the MT protein does provide a defence mechanism to protect cells from monofunctional alkylating and cross-linking agents but not from free radicals.

Citations

Oct 31, 2000·The Journal of Pathology·A M JanssenH W Verspaget
Sep 5, 2002·Cancer·Hanifa BouzourenePhilippe Coucke
Oct 1, 1992·Cell Biology and Toxicology·P MoffattF Denizeau
Nov 1, 1990·Somatic Cell and Molecular Genetics·J Koropatnick, J Pearson
Jan 1, 1991·Archives of Toxicology·T MüllerL Jaenicke
Jan 1, 1992·Archives of Toxicology·M SatohN Imura
Sep 1, 1991·Biological Trace Element Research·M E LeibrandtM G Cherian
Sep 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·K H TempelI Stammberger
Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Jun 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J R MastersH Lohrer
Jun 19, 2001·Mutation Research·M Kartalou, J M Essigmann
Nov 14, 1997·Mechanisms of Ageing and Development·C A WalterA Richardson
Mar 4, 2000·Free Radical Biology & Medicine·C C ConradA Richardson
Nov 13, 2008·Cytotechnology·G Damia, M D'Incalci
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M L Chernaik, P C Huang
Apr 1, 1995·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T HaerslevK Zedeler
May 20, 1999·Annual Review of Pharmacology and Toxicology·C D KlaassenS Choudhuri
Jan 1, 1996·Cancer Investigation·C P SchröderR F Ozols
Oct 12, 2010·Ageing Research Reviews·William R Swindell
Jan 1, 1992·Current Problems in Cancer·T C Hamilton
Nov 2, 2006·The Journal of Toxicological Sciences·Nobuhiko MiuraAkira Naganuma
Jan 15, 1993·Cancer·S W JohnsonT C Hamilton
Oct 5, 2006·Experimental Biology and Medicine·Boon-Huat BayPuay-Hoon Tan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.