Overexpression of methionine sulfoxide reductases A and B2 protects MOLT-4 cells against zinc-induced oxidative stress

Antioxidants & Redox Signaling
Filipe CabreiroIsabelle Petropoulos

Abstract

Among the amino acids, methionine is the most susceptible to oxidation, and methionine sulfoxide can be catalytically reduced within proteins by methionine sulfoxide reductase A (MsrA) and B (MsrB). As one of the very few repair systems for oxidized proteins, MsrA and MsrB enzymes play a major role in protein homeostasis during aging and have also been involved in cellular defenses against oxidative stress, by scavenging reactive oxygen species. To elucidate the role of zinc on the Msr system, the effects of zinc treatment on control and stably overexpressing MsrA and MsrB2 MOLT-4 leukemia cells have been analyzed. Here we show that zinc treatment has a pro-antioxidant effect in MOLT-4 cells by inducing the transcription of metallothioneins and positively modulating the activity of the Msr enzymes. In contrast, due to its pro-oxidant effect, zinc also led to increased cell death, reactive oxygen species production, and protein damage. Our results indicate that overexpression of the Msr enzymes, due to their antioxidant properties, counteracts the pro-oxidant effects of zinc treatment, which lead to a cellular protection against protein oxidative damage and cell death, by reducing the production of reactive oxygen species.

References

Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·R L LevineE R Stadtman
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·J MoskovitzE R Stadtman
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J MoskovitzE R Stadtman
Dec 23, 1999·The Journal of Biological Chemistry·A LescureA Krol
May 10, 2000·The Journal of Nutrition·S R Powell
May 20, 2000·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·I PetropoulosB Friguet
Dec 1, 2000·Journal of Biochemistry·M HamatakeR Ishida
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J MoskovitzE R Stadtman
Oct 26, 2001·The Journal of Biological Chemistry·R GrimaudF Barras
Feb 14, 2002·Archives of Biochemistry and Biophysics·Herbert WeissbachNathan Brot
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hongyu RuanStephen H Heinemann
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alfred HanselStefan H Heinemann
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·William B Bonvillian
Jul 30, 2002·The Journal of Biological Chemistry·R Abhilash KumarVadim N Gladyshev
Oct 29, 2003·Antioxidants & Redox Signaling·Earl R StadtmanRodney L Levine
Dec 31, 2003·Molecular Biology of the Cell·Hwa-Young Kim, Vadim N Gladyshev
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Olena YermolaievaToshinori Hoshi
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Marc KantorowJ F Hejtmancik
Feb 1, 2005·Biochimica Et Biophysica Acta·Michael J Davies
Feb 1, 2005·Biochimica Et Biophysica Acta·Herbert WeissbachNathan Brot
May 26, 2005·Investigative Ophthalmology & Visual Science·Maria A MarchettiMarc Kantorow
Jun 1, 2005·Toxicology and Applied Pharmacology·Mi Ja ChungChrister Hogstrand
Nov 1, 2005·Free Radical Biology & Medicine·Cédric R PicotBertrand Friguet
Nov 26, 2005·Journal of Alzheimer's Disease : JAD·Qiang Hao, Wolfgang Maret
Mar 18, 2006·BMC Molecular Biology·Hwa-Young Kim, Vadim N Gladyshev
Sep 22, 2006·Antioxidants & Redox Signaling·Wolfgang Maret
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kristine M RobinsonJoseph S Beckman
Oct 10, 2006·Biogerontology·V FrazziniS L Sensi
Feb 15, 2007·Biochemistry·James S Butler, Stewart N Loh
Jul 12, 2007·Molecular Medicine·Wolfgang Maret, Artur Krezel
Aug 25, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Alessia FormigariAlessandro Santon
Sep 1, 2007·Antioxidants & Redox Signaling·Alla V Glushchenko, Donald W Jacobsen
Oct 10, 2007·The Biochemical Journal·Hwa-Young Kim, Vadim N Gladyshev

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Citations

Dec 25, 2012·Environmental Science and Pollution Research International·Wenguang XiongRizwan-Ul-Haq Muhammad
Dec 5, 2009·Antioxidants & Redox Signaling·Nicolas UgarteBertrand Friguet
Mar 23, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sarah D Lamore, Georg T Wondrak
Oct 31, 2012·Molecular Aspects of Medicine·Niki ChondrogianniEfstathios S Gonos
Nov 17, 2011·Biochemical and Biophysical Research Communications·Seung Hee Choi, Hwa-Young Kim
Mar 17, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Chenglong WuXiaoli Zhong
Oct 26, 2010·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero
May 2, 2009·Biochimica Et Biophysica Acta·Byung Cheon LeeVadim N Gladyshev
Aug 31, 2013·Free Radical Biology & Medicine·Nicolas UgarteIsabelle Petropoulos
Aug 28, 2020·Metallomics : Integrated Biometal Science·Laurent ChavatteAnne-Laure Bulteau
Jul 19, 2020·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero

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