Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.

Cell Cycle
Bing WenJiaxuan Qiu

Abstract

microRNAs have been proved to function in some processes of differentiation and the effect is favorable. At present, the differentiation of stem cells is not so ideal because of the high expenses and inaccessibility. Therefore, we explored the possibility that microRNA-221 (miR-221) affects differentiation from stem cells from human deciduous tooth (SHEDs) to neurons through Wnt/β-catenin pathway via binding to CHD8. After collection of SHEDs, differentiation from SHEDs to neurons was conducted by neurotrophic factor induction method in vitro, followed by gain- and loss-of-function experiments. Expression of neuron-related genes in SHEDs was examined by immunohistochemistry. The relationship between CHD8 and miR-221 was detected by dual luciferase reporter gene assay. RT-qPCR and Western blot analysis were used to determine miR-221 expression, and the mRNA and protein expression of CHD8, Wnt/β-catenin pathway- and neuron-related genes. Cell viability, and cell cycle and apoptosis were investigated by MTT assay and flow cytometry respectively. Dual luciferase reporter assay displayed that miR-221 targeted CHD8 and then affected the differentiation progression. Results of RT-qPCR and Western blot analysis showed that expression o...Continue Reading

References

Nov 16, 2011·Molecular and Cellular Biology·Masaaki NishiyamaKeiichi I Nakayama
Jul 23, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Wen-Wen ChouSuh-Hang Hank Juo
May 31, 2014·Canadian Journal of Physiology and Pharmacology·Fang-Tian XuKun-Ming Xu
Dec 6, 2014·Journal of Cellular and Molecular Medicine·Punitha VasanthanNoor Hayaty Abu Kasim
Jul 30, 2015·BioMed Research International·Dmitry A ChistiakovYuri V Bobryshev
Nov 5, 2015·Advances in Experimental Medicine and Biology·Maria Antonietta IsgròRoberto Scatena
Dec 3, 2015·Molecular Cell·Chen ShenChristopher R Vakoc
Jan 7, 2016·Frontiers in Neuroscience·Rebecca A BarnardBrian J O'Roak
Mar 22, 2016·The Spine Journal : Official Journal of the North American Spine Society·Ching-Hua YehXudong Joshua Li
Jan 27, 2017·PloS One·Karlen G Gazarian, Luis R Ramírez-García
Apr 14, 2017·Cell Reports·Randall J PlattFeng Zhang
Jun 16, 2017·Journal of Tissue Engineering·Elna Paul ChalisserrySukumaran Anil
Nov 4, 2017·Cell Death and Differentiation·Younseo OhJungwook Hwang
Jan 18, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazunori WatanabeTakashi Ohtsuki
Feb 27, 2018·International Journal of Stem Cells·Nathaphon TangjitHathaitip Sritanaudomchai
Mar 13, 2018·Stem Cell Reports·Roberto De GregorioGian Carlo Bellenchi
Jun 14, 2018·Journal of Biomedical Materials Research. Part a·Jelena SimonovicJelena Milasin
Jan 19, 2019·Methods in Molecular Biology·Hyenjong Hong, Marcel M Daadi
Oct 11, 2019·Archives of Oral Biology·Thulasi Thiruvallur MadanagopalVinicius Rosa

❮ Previous
Next ❯

Citations

Nov 7, 2021·Cellular and Molecular Life Sciences : CMLS·Rohan Gupta Pravir Kumar

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR
electrophoresis
transfection

Software Mentioned

SPSS

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.