Overexpression of MicroRNA-29b Decreases Expression of DNA Methyltransferases and Improves Quality of the Blastocysts Derived from Somatic Cell Nuclear Transfer in Cattle

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Shuang LiangXiang-Shun Cui

Abstract

MicroRNA (miR)-29b plays a crucial role during somatic cell reprogramming. The aim of the current study was to explore the effects of miR-29b on the developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos, as well as the underlying mechanisms of action. The expression level of miR-29b was lower in bovine SCNT embryos at the pronuclear, 8-cell, and blastocyst stages compared with in vitro fertilized embryos. In addition, miR-29b regulates the expression of DNA methyltransferases (Dnmt3a/3b and Dnmt1) in bovine SCNT embryos. We further investigated SCNT embryo developmental competence and found that miR-29b overexpression during bovine SCNT embryonic development does not improve developmental potency and downregulation inhibits developmental potency. Nevertheless, the quality of bovine SCNT embryos at the blastocyst stage improved significantly. The expression of pluripotency factors and cellular proliferation were significantly higher in blastocysts from the miR-29b overexpression group than the control and downregulation groups. In addition, outgrowth potential in blastocysts after miR-29b overexpression was also significantly greater in the miR-29b overexpression group than in the control and downregula...Continue Reading

References

Mar 7, 1996·Nature·K H CampbellI Wilmut
Aug 6, 2000·Fertility and Sterility·B BalabanR Mercan
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·W DeanW Reik
Jan 5, 2002·Molecular and Cellular Biology·Gangning LiangPeter A Jones
Dec 17, 2005·Biochemical and Biophysical Research Communications·Satoshi KishigamiTeruhiko Wakayama
Sep 1, 2006·Biology of Reproduction·Manami AmanaiAnthony C F Perry
Sep 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Heiner NiemannRita S F Lee
Jan 30, 2009·Cell Cycle·Alicia Barroso-del JesusPablo Menendez
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Feb 3, 2011·The EMBO Journal·Zhonghan LiTariq M Rana
Apr 9, 2011·Cell Stem Cell·Frederick Anokye-DansoEdward E Morrisey
Sep 24, 2011·Trends in Genetics : TIG·Vincent PasqueJ B Gurdon
Dec 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wei-Min LiuWilliam S B Yeung
Feb 1, 2012·Biochemical and Biophysical Research Communications·Gang LiYuxiang Chen
Sep 11, 2012·Journal of Cellular Biochemistry·Chih-Hao Kuo, Shao-Yao Ying
Apr 16, 2013·Development Genes and Evolution·Xin LiuYi-Quan Wang
Nov 5, 2014·Medical Oncology·Li Li LinHanGuang Qian
Mar 15, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Junqiang ZhangChang Liu
May 21, 2016·Cancer Research·Stephanie LangschErik Vassella
Jul 28, 2016·Scientific Reports·Sheng ZhangZiyi Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.