Overexpression of microRNA‑101 causes anti‑tumor effects by targeting CREB1 in colon cancer

Molecular Medicine Reports
Qinglin YangXiaoli Han

Abstract

Accumulating evidence has demonstrated that aberrantly expressed microRNAs (miRNAs) are involved in the initiation and progression of numerous types of human cancer. Although a number of miRNAs have been demonstrated to be associated with the diagnosis, progression and prognosis of colon cancer, the function of miRNA‑101 (miR‑101) in colon cancer remains unclear, and the molecular mechanisms underlying the effects of miR‑101 in colon cancer require further investigation. The present study investigated the role of miR‑101 in colon cancer, and the results suggested that miR‑101 expression levels were significantly decreased in colorectal carcinoma tissues and in three types of colorectal cancer cell lines. Furthermore, overexpression of miR‑101 inhibited cell proliferation and migration in HT29 cells. The transcription factor cAMP responsive element binding protein 1 (CREB1) was identified to be a direct target of miR‑101 using a luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction analysis and western blot assay. miR‑101 overexpression in tumor xenografts in vivo decreased the expression levels of proliferating cell nuclear antigen and CREB1, and suppressed tumor growth. The present results sug...Continue Reading

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Citations

Feb 6, 2020·International Journal of Molecular Sciences·Giacomo SettiMarco Meleti
Mar 12, 2020·Anti-cancer Agents in Medicinal Chemistry·Poonam VermaSaurav Sarkar
Aug 22, 2020·Cancer Management and Research·Yuhui LiGuomin Huang
Aug 28, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Shruthi Sanjitha SampathSatish Ramalingam

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Methods Mentioned

BETA
surgical resection
transfection
Assay
Protein Assay
electrophoresis
xenograft

Software Mentioned

TargetScan
Pro Plus
Image
Quantity One

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