Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2

Scientific Reports
Yingfeng TuBaozhong Shen

Abstract

Recent studies reported miR-497 exhibited inhibitory effects in various cancers. However, whether miR-497 is involved in inhibiting angiogenesis, which is critical for tumor growth and metastasis, is still unknown. The purpose of this study was to investigate the potential role of miR-497 in tumor angiogenesis. In this work, cell proliferation and apoptosis analyses were conducted to explore the potential function of miR-497 in HUVECs by using MTT and TUNEL assays. Western blotting (WB) was employed to validate the downstream targets of miR-497. Furthermore, in order to disclose the role of miR-497 on angiogenesis, VEGFR2-luc transgenic mice were treated with miR-497 mimic and applied to monitor tumor angiogenesis and growth by in vivo bioluminescent imaging (BLI). The results demonstrated that overexpression of miR-497 showed inhibitory effects on VEGFR2 activation and downstream Raf/MEK/ERK signal pathways in vitro and in vivo. Moreover, overexpression of miR-497 effectively induced HUVECs apoptosis by targeting VEGFR2 and downstream PI3K/AKT signaling pathway. Furthermore, miR-497 exhibited anti-angiogenesis and anti-tumor effects in the VEGFR2-luc breast tumor model proven by BLI, WB and immunohistochemistry analysis. In su...Continue Reading

References

Apr 19, 2005·Nature Medicine·Jean-Sébastien SilvestreZiad Mallat
Jun 10, 2005·Nature·Jun LuTodd R Golub
Dec 16, 2005·Nature·Leigh CoultasJanet Rossant
Mar 23, 2006·Cell Cycle·Payaningal R SomanathTatiana V Byzova
Oct 31, 2006·The American Journal of Pathology·Danielle A MurphyWilliam M F Lee
Oct 31, 2006·Oncogene·M-L SiY-Y Mo
Jun 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eric L KaijzelClemens W G M Löwik
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel Y LeeBurton B Yang
Mar 29, 2008·Japanese Journal of Ophthalmology·Yasuaki HataTatsuro Ishibashi
Aug 30, 2008·Current Opinion in Pharmacology·Timothy A YapPaul Workman
Feb 26, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dan LiLihui Lai
Sep 1, 2011·The Journal of Biological Chemistry·Sanjay YadavDevendra Parmar
Nov 25, 2011·Current Cancer Drug Targets·A AminiM H Pourgholami
Dec 6, 2011·Journal of Molecular and Cellular Cardiology·Xiaojun LiuChunxiang Zhang
May 2, 2012·The AAPS Journal·Stacey D Finley, Aleksander S Popel
Sep 13, 2012·Experimental and Therapeutic Medicine·Lei ShenLin Fang
Oct 25, 2012·FEBS Letters·Dongling ZhengPatricia Woo
Jan 1, 2013·Anti-cancer Agents in Medicinal Chemistry·Paul W Sylvester, Nehad M Ayoub
Feb 15, 2013·Thrombosis and Haemostasis·Josef EhlingFabian Kiessling
May 15, 2013·Molecular and Cellular Endocrinology·Alice Y S Law, Chris K C Wong
May 16, 2013·Biochemical and Biophysical Research Communications·Wen-yan ZhaoPeng Chen

❮ Previous
Next ❯

Citations

Dec 3, 2015·Journal of Cellular Physiology·Gaetano Santulli
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yawen MaYunhui Liu
Aug 3, 2018·Journal of Cellular Physiology·Nasser H GoradelAmirhossein Sahebkar
Feb 6, 2020·Biomolecules·Elizabeth VargheseDietrich Büsselberg
Feb 1, 2018·Oncotarget·Weiyang LouWeimin Fan
Jun 28, 2016·Oncotarget·Gang YangYupei Zhao
Oct 1, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xianjie JiangWei Xiong
Oct 27, 2018·Current Clinical Pharmacology·Keywan MortezaeeBagher Farhood
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Nov 30, 2018·International Journal of Molecular Medicine·Yarely M Salinas-VeraCésar López-Camarillo
Jul 3, 2021·International Journal of Molecular Sciences·Maliha Tabassum MunirShaikh Mizanoor Rahman
Aug 7, 2019·Seminars in Cancer Biology·Francesca OrsoDaniela Taverna

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
xenograft
transgenic
Assay
reverse transcription-PCR
protein assay
Infrared Imaging

Software Mentioned

ImagePro Plus
Living Image
Living Image®
Odyssey
TargetScan

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.