Overexpression of Muscadinia rotundifolia CBF2 gene enhances biotic and abiotic stress tolerance in Arabidopsis

Protoplasma
Jiao WuYali Zhang

Abstract

C-repeat-binding factor dehydration-responsive element-binding factor 1C (CBF2/DREB1C) gene encodes a small family of transcriptional activator that has been described as playing an important role in freezing tolerance and cold acclimation of plants. We here report that CBF2 gene also plays an important role in the early response to the pathogen infection of grapevine downy mildew disease. The expression level of CBF2 increased dramatically and reached a peak at 7 h after infection in immune grapevine Muscadinia rotundifolia 'Noble', which was much faster than moderate resistant Vitis amurensis 'PI1288' and susceptible Vitis vinifera 'Cabernet Sauvignon'. Muscadinia rotundifolia MrCBF2 exhibited amino acid domains characteristic of Vitis CBF2 proteins with unique features including rich serine repeats and slight differences in NLS, DSAWRL, and AP2 domains. The MrCBF2 gene was introduced to Arabidopsis 'COL0' which are susceptible to downy mildew pathogen. The transgenic lines showed an increased resistance to downy mildew disease and more accumulation of SA as well as higher expression of pathogenesis-related (PR) genes (AtPR1, AtPR4, and AtPR5) as a consequence of MrCBF2 overexpression. Besides, constitutive expression of MrCB...Continue Reading

References

Jul 1, 1997·Genes & Development·Y YangD F Klessig
Feb 27, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N P HarberdD E Richards
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jan 19, 2002·Biochemical and Biophysical Research Communications·Yoh SakumaKazuko Yamaguchi-Shinozaki
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Sep 12, 2002·Plant Physiology·K. Shinozaki, K. Yamaguchi-Shinozaki
Oct 12, 2002·Plant Physiology·Volker HaakeJames Z Zhang
Mar 22, 2003·Science·Nicholas P Harberd
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Jeffrey Leung, Jerome Giraudat
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·J. Ingram, D. Bartels
May 18, 2004·The Plant Journal : for Cell and Molecular Biology·Andrea ChiniGary J Loake
Jul 3, 2004·Current Opinion in Plant Biology·Neal Gutterson, T Lynne Reuber
Sep 10, 2004·Plant Molecular Biology·Sarah J GilmourMichael F Thomashow
Jan 7, 2005·The Plant Journal : for Cell and Molecular Biology·Jonathan T VogelMichael F Thomashow
Mar 8, 2005·Trends in Plant Science·Mansour KarimiPierre Hilson
Oct 26, 2005·Plant Molecular Biology·Jeffrey S SkinnerPatrick M Hayes
Nov 4, 2006·Plant, Cell & Environment·Huogen XiaoAnnette Nassuth
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fernando NovilloJulio Salinas
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Joaquín MedinaJulio Salinas
Sep 3, 2011·The Plant Journal : for Cell and Molecular Biology·Takayuki AsanoRyu Ohsugi

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Citations

Feb 9, 2017·Frontiers in Plant Science·Tao WeiYong Zhang

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BETA
transgenic
PCR
gene
confocal microscopy

Software Mentioned

StepOne [UNK]
BLAST
ClustalW2
SPSS
Masslynx
DNAman

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