Overexpression of mutated forms of aspartate kinase and cystathionine gamma-synthase in tobacco leaves resulted in the high accumulation of methionine and threonine

The Plant Journal : for Cell and Molecular Biology
Yael HachamRachel Amir

Abstract

Methionine and threonine are two essential amino acids, the levels of which limit the nutritional quality of plants. Both amino acids diverge from the same branch of the aspartate family biosynthesis pathway; therefore, their biosynthesis pathways compete for the same carbon/amino substrate. To further elucidate the regulation of methionine biosynthesis and seek ways of increasing the levels of these two amino acids, we crossed transgenic tobacco plants overexpressing the bacterial feedback-insensitive aspartate kinase (bAK), containing a significantly higher threonine level, with plants overexpressing Arabidopsis cystathionine gamma-synthase (AtCGS), the first unique enzyme of methionine biosynthesis. Plants co-expressing bAK and the full-length AtCGS (F-AtCGS) have significantly higher methionine and threonine levels compared with the levels found in wild-type plants, but the methionine level does not increase beyond that found in plants expressing F-AtCGS alone. This finding can be explained through the feedback inhibition regulation mediated by the methionine metabolite on the transcript level of AtCGS. To test this assumption, plants expressing bAK were crossed with plants expressing two mutated forms of AtCGS in which the...Continue Reading

References

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Citations

Apr 8, 2010·Amino Acids·R A Azevedo, P Arruda
Jun 1, 2012·BMC Plant Biology·Yongrui WuJoachim Messing
Aug 13, 2014·Journal of Experimental Botany·Réjane Pratelli, Guillaume Pilot
Jan 7, 2016·Annual Review of Plant Biology·Gad GaliliAlisdair R Fernie
Apr 4, 2013·International Journal of Molecular Sciences·Lyuben ZagorchevMariela Odjakova
Jun 21, 2017·International Journal of Molecular Sciences·Guoping WangGad Galili
Apr 29, 2018·Plant Biotechnology Journal·Zetao BaiChunxiang Fu
Feb 6, 2018·Plant Reproduction·Wenyi WangGad Galili
Mar 27, 2019·Journal of Experimental Botany·Rachel AmirYael Hacham
Sep 4, 2018·Bioscience, Biotechnology, and Biochemistry·Tatsuya OhshidaHaruhiko Sakuraba

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