Overexpression of MzASMT 1 , a Gene From Malus zumi Mats, Enhances Salt Tolerance in Transgenic Tobacco

Frontiers in Plant Science
Weibing ZhuangShenchun Qu

Abstract

Melatonin, widely found in various plants as a new antioxidant, could protect plants from various biotic and/or abiotic stresses, including salt stress. MzASMT 1 (KJ123721), a gene from Malus zumi Mats, is a key enzyme required for melatonin synthesis. However, whether the overexpression of MzASMT 1 could regulate the synthesis of melatonin and improve the salt tolerance in tobacco remains unknown. In this study, the overexpression of MzASMT 1 in tobacco increased the melatonin content, and the transgenic lines owned higher salt tolerance capacity. The transgenic lines overexpressing MzASMT 1 exhibited lower degree of leaf wilting; much more fresh weight; higher plant height; longer root; higher relative water content (RWC) of leaves, stem, and root; and higher chlorophyll content and Fv/Fm, which makes transgenic lines better adapt to salt stress. The transgenic lines also had higher accumulation of proline, lower accumulation of malondialdehyde (MDA), and improved antioxidant systems, which protected plants from cell damage and oxidative stress due to excess reactive oxygen species (ROS) accumulation under salt treatment. The transcription of salt response genes was much more highly activated in transgenic lines than in wild ...Continue Reading

References

Feb 15, 2001·Trends in Plant Science·J K Zhu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·James F DatFrank Van Breusegem
Jul 3, 2004·Planta·Josefa Hernández-RuizMarino B Arnao
Aug 16, 2005·Journal of Pineal Research·Josefa Hernández-RuizMarino B Arnao
Dec 21, 2006·Biochemical and Biophysical Research Communications·Ming ChenYou-Zhi Ma
Feb 9, 2007·Journal of Pineal Research·Marino B Arnao, Josefa Hernández-Ruiz
May 23, 2007·Journal of Dairy Science·F-Q Zhao, A F Keating
Aug 12, 2008·Journal of Pineal Research·M B Arnao, J Hernández-Ruiz
Feb 7, 2009·Journal of Pineal Research·Marino B Arnao, Josefa Hernández-Ruiz
May 1, 2006·Plant Signaling & Behavior·Marino B Arnao, Josefa Hernández-Ruiz
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Sep 3, 2011·Journal of Pineal Research·Carmen VenegasDarío Acuña-Castroviejo
Oct 22, 2011·Journal of Experimental Botany·Dun-Xian TanRussel J Reiter
Sep 25, 2012·Journal of Pineal Research·Annia GalanoRussel J Reiter
Sep 29, 2012·Plant Science : an International Journal of Experimental Plant Biology·Junzhi DuanZichao Li
Oct 31, 2012·Journal of Pineal Research·Ping WangFengwang Ma
Mar 14, 2013·Journal of Pineal Research·Yu ZhaoJin Kong
Jul 28, 2013·Plant Signaling & Behavior·Aparna Krishnamurthy, Bala Rathinasabapathi
Sep 17, 2013·Journal of Pineal Research·Sangkyu ParkKyoungwhan Back
Oct 19, 2013·Journal of Pineal Research·Yeong ByeonKyoungwhan Back
Aug 12, 2014·BioMed Research International·Mirza HasanuzzamanMasayuki Fujita
Oct 10, 2014·Plant Physiology and Biochemistry : PPB·Shumei ZhouWei Wang
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Russel J ReiterAnnia Galano
Apr 29, 2015·Journal of Pineal Research·Chengzhen LiangChengcai Chu
May 31, 2017·Plant Science : an International Journal of Experimental Plant Biology·Quan GuWenbiao Shen
Jun 21, 2017·Plant Physiology and Biochemistry : PPB·Guibin CuiYajun Xi
Oct 27, 2017·Annals of Botany·M B Arnao, J Hernández-Ruiz
Nov 13, 2017·Biochemical and Biophysical Research Communications·Wenji LiangLianyin Liu

❮ Previous
Next ❯

Datasets Mentioned

BETA
KJ123721

Methods Mentioned

BETA
salt treatment
transgenic
MDA
enzyme-linked immunosorbent assay
PCR
environmental stresses

Software Mentioned

Excel
GraphPad Prism
DNAMAN
Primer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.