Overexpression of N-WASP in the brain of human epilepsy

Brain Research
Fei XiaoJie Yuan

Abstract

Neuronal circuit remodeling is the most critical pathological characteristic closely associated with the initiation and maintenance of epilepsy; however, the exact mechanisms of neuronal remodeling need further elucidation. Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is a key regulator of the actin cytoskeleton that causes actin polymerization and thus neurite extension. Our previous research demonstrated that the upstream regulator of N-WASP, cell division cycle 42 GTP-binding protein (Cdc42), is significantly upregulated in the brains of patients with intractable epilepsy (IE). In addition, cDNA microarray analysis has shown that gene expression of N-WASP is notably enhanced in the epileptic brain, suggesting a possible role for N-WASP in epileptogenesis. Here, we investigated the expression of N-WASP and its downstream effector, actin-related protein 2/3 (Arp2/3), at the protein level in the temporal lobe of IE patient brains to explore its possible role in the genesis of IE. Forty surgical samples from brains of patients with IE and 20 control brain tissues were obtained for this study. The expression of N-WASP in the anterior temporal neocortex was detected using immunohistochemistry, immunofluorescence and western ...Continue Reading

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Citations

Feb 9, 2010·Journal of Molecular Neuroscience : MN·Jie YuanXue-feng Wang
Apr 7, 2011·Experimental & Molecular Medicine·Chang Hoon Cho
Jul 26, 2011·Neurochemical Research·Xi PengXuefeng Wang
Dec 17, 2011·The International Journal of Biochemistry & Cell Biology·Chen ChenEvgeni Ponimaskin
Mar 25, 2011·Medical Hypotheses·Min FangXue-Feng Wang
Nov 29, 2011·Experimental Neurology·Yanbing HanGuojun Chen
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Mar 14, 2018·Annals of Neurology·Mitsuko NakashimaNaomichi Matsumoto
Dec 12, 2020·The European Journal of Neuroscience·Iman Imtiyaz Ahmed Juvale, Ahmad Tarmizi Che Has
Apr 20, 2021·Frontiers in Molecular Biosciences·Matilde GhibaudiAlessandro Vercelli
Jun 17, 2021·Chemical Biology & Drug Design·Nadia PerveenImran Imran

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