Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer

BMC Urology
Yan-Ru ZengWei-de Zhong

Abstract

The NIMA-related kinase 2 (NEK2) is a serine/threonine kinase that is involved in regulation of centrosome duplication and spindle assembly during mitosis. Dysregulation of these processes causes chromosome instability and aneuploidy, which are hallmark changes of many solid tumors. However, whether aberrant expression of NEK2 is associated with outcome of prostate cancer (PCa) patients remains to be determined. Expression of NEK2 in human PCa cells and primary PCa tissues was assessed by quantitative RT-PCR. Expression of NEK2 in human PCa cells was depleted with siRNA. Effects of the depletion on cell proliferation, survival, and tumorigenicity were assessed both in vitro with cell cultures and in vivo with subcutaneous implantation of xenografts. In silico analyses of the online Taylor dataset were carried out to determine whether the expression level of NEK2 correlated with the clinicopathological characteristics of prostate cancer. Compared with benign human prostatic epithelial cells and tissues, the expression of NEK2 was elevated in human PCa cells and primary PCa tissues. Depleting NEK2 expression inhibited human PCa cell proliferation in vitro and xenograft growth in vivo. Expression level of NEK2 in PCa positively co...Continue Reading

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Citations

Mar 29, 2016·Cell Cycle·Yanfen Fang, Xiongwen Zhang
Dec 1, 2017·International Journal of Molecular Sciences·Marilina García-Aranda, Maximino Redondo
Nov 12, 2016·Oncotarget·Christopher R CoxonCéline Cano
Jun 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Honghong ShenChen Wang
Sep 1, 2017·Journal of Cellular Biochemistry·Mehdi ArianaMohammad Esmaeil Akbari
Jan 8, 2020·BMC Cancer·Talita Diniz Melo-HanchukJörg Kobarg
Nov 18, 2020·Journal of Physiology and Biochemistry·Hao WanTaiyuan Li

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Methods Mentioned

BETA
transfection
xenograft
xenografts

Software Mentioned

SAS
SPSS
Opticon Monitor

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