Overexpression of OsbHLH107, a member of the basic helix-loop-helix transcription factor family, enhances grain size in rice (Oryza sativa L.)

Rice
Xiaoming YangJianmin Wan

Abstract

Grain size, which is determined by grain length, grain width, and grain thickness, is an important determinant for grain yield in rice. Identification and characterization of new genes that are associated with grain size will be helpful for the improvement of grain yield in rice. We characterized the grain size mutant, larger grain size 1 (lgs1), derived from rice activation-tagged T-DNA insertion lines. Histological analysis showed that increased cell numbers in the longitudinal direction of spikelet hulls was responsible for the grain mutant phenotype in lgs1. Quantitative real-time PCR (qRT-PCR) analysis further showed that the expression levels of genes associated with the cell cycle in the young panicles of the lgs1 were higher than those in the wild type (WT), which might result in the increased cell numbers in lgs1 spikelet hulls. Insertion site analysis together with transgenic experiments confirmed that the lgs1 phenotype was caused by enhanced expression of truncated OsbHLH107, corresponding to the nucleotide (nt) 331-846 region (i.e., the transcriptional activation region of OsbHLH107) of the OsbHLH107 coding sequence (CDS). OsbHLH107 is a nucleus-localized bHLH transcription factor, which can form a homodimer with i...Continue Reading

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Citations

Feb 20, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jianyu WenShaoqing Li
Jul 16, 2019·Critical Reviews in Biotechnology·P AziziM N Yusuf
Nov 30, 2019·Frontiers in Plant Science·Vívian Ebeling VianaAntonio Costa de Oliveira
Mar 22, 2020·International Journal of Molecular Sciences·Hyoseob SeoNam-Chon Paek
Aug 8, 2021·International Journal of Molecular Sciences·Seonghoe JangYue-Ie Hsing

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Methods Mentioned

BETA
PCR
transgenic
two-hybrid
Y2H

Software Mentioned

DNAMAN
Primer Premier
BLAST
ImageJ
GenScript
MEGA

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