Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice.

Plant Molecular Biology
Vydehi Kanneganti, Aditya Kumar Gupta

Abstract

We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submergence, wounding, heavy metals as well as stress hormone Abscisic acid. OsiSAP8 protein fused to GFP was localized towards the periphery of the cells in the epidermal cells of infiltrated Nicotiana benthamiana leaves. Yeast two hybrid analysis revealed that A20 and AN1 type zinc-finger domains of OsiSAP8 interact with each other. Overexpression of the gene in both transgenic tobacco and rice conferred tolerance to salt, drought and cold stress at seed germination/seedling stage as reflected by percentage of germination and gain in fresh weight after stress recovery. Transgenic rice plants were tolerant to salt and drought during anthesis stage without any yield penalty as compared to unstressed transgenic plants.

References

Jan 1, 1992·Molecular Plant-microbe Interactions : MPMI·Y K SharmaM C Mehdy
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1993·Plant Physiology·K Yamaguchi-Shinozaki, K Shinozaki
Jul 26, 1996·The Journal of Biological Chemistry·J T CooperC Ferran
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·Y SaijoK Izui
Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·B L OrvarR S Dhindsa
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·C LlaveJ C Carrington
Dec 15, 2000·The Plant Journal : for Cell and Molecular Biology·A OberschallG V Horváth
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Utako YamanouchiKyoji Yamada
Sep 12, 2002·Plant Physiology·K. Shinozaki, K. Yamaguchi-Shinozaki
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Sean D SimpsonKazuko Yamaguchi-Shinozaki
Feb 3, 2004·The Journal of Biological Chemistry·Jun HuangHong-Bing Shu
Apr 10, 2004·Journal of Experimental Botany·Huixia ShouKan Wang
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Arnab MukhopadhyayAkhilesh K Tyagi
Jul 1, 2004·Molecular Cell·Markus TeigeHeribert Hirt
Apr 16, 2005·Current Opinion in Biotechnology·Basia Vinocur, Arie Altman
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Jan 21, 2006·The EMBO Journal·Akinori HishiyaKen Watanabe
Feb 8, 2006·Nature Structural & Molecular Biology·Sangho LeeJames H Hurley
Feb 18, 2006·Current Opinion in Plant Biology·Babu Valliyodan, Henry T Nguyen

❮ Previous
Next ❯

Citations

Dec 7, 2010·Plant Cell Reports·Toto Hadiarto, Lam-Son Phan Tran
Sep 11, 2012·Plant Molecular Biology·Shareena SreedharanThumballi R Ganapathi
Feb 8, 2011·Plant Physiology and Biochemistry : PPB·Christine Gimeno-GillesEric Lelievre
May 4, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jitender GiriAkhilesh K Tyagi
Feb 9, 2011·Biotechnology Progress·Syed Sarfraz HussainMuhammad Amjad
Apr 18, 2016·Plant Physiology and Biochemistry : PPB·Zala KolencAndreja Čerenak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.