Overexpression of P2Y2 purinoceptor in intimal lesions of the rat aorta

Arteriosclerosis, Thrombosis, and Vascular Biology
Cheikh I SeyeC Desgranges

Abstract

Extracellular nucleotides, particularly ATP, are involved in the modulation of arterial vasomotricity via P2 purinoceptors present on smooth muscle and endothelial cells. These nucleotides could also be implicated in the smooth muscle cell hyperplasia observed in intimal lesions. In this study, we tried to define the potential role of the P2Y2 (P2u) purinoceptor by studying its expression in normal and balloon-injured rat aortas. The cloning of a rat P2Y2 cDNA from a rat smooth muscle cell cDNA library made it possible to study P2Y2 expression both by Northern blot and in situ hybridization. Northern blot experiments indicated that P2Y2 mRNA was present in rat medial aortic smooth muscle and in cultured rat aortic smooth muscle cells. In situ hybridization indicated that P2Y2 mRNA was present in endothelial cells of the intima and in some smooth muscle cells scattered throughout the media of adult rat aortas, while almost all medial smooth muscle cells of rat embryo aorta expressed this receptor. In contrast with adult aortic media, the majority of neointimal smooth muscle cells found in aortic intimal lesions either 8 or 20 days after balloon injury were positive for P2Y2 mRNA. Moreover, a subpopulation of neointimal cells loc...Continue Reading

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Citations

Dec 19, 2001·Archives of Biochemistry and Biophysics·Katrin Sak, Tania E Webb
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Laurie ErbGary A Weisman
Apr 12, 2008·Purinergic Signalling·Cheikh I SeyeGary A Weisman
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