Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells

OncoTargets and Therapy
Qiangqiang GeZhangqun Ye

Abstract

Previous research has reported that a particular double-stranded RNA, named dsP53-285, has the capacity to induce expression of the tumor suppressor gene TP53 in chimpanzee cells by targeting its promoter. Usually, it is the wild-type p53 protein, rather than mutants, which exhibits potent cancer-inhibiting effects. In addition, nonhuman primates, such as chimpanzees, share almost identical genome sequences with humans. This prompted us to speculate whether dsP53-285 can trigger wild-type p53 protein expression in human prostate cancer (PCa) cells and consequently suppress cell growth. The human PCa cell lines LNCaP and DU145 were transfected with dsP53-285 for 72 hours. Compared with the dsControl and mock transfection groups, expression of both p53 messenger RNA and p53 protein was significantly enhanced after dsP53-285 transfection, and this enhancement was followed by upregulation of p21, which indirectly indicated that dsP53-285 induced wild-type p53 expression. Moreover, overexpression of wild-type p53 mediated by dsP53-285 downregulated the expression of Cyclin D1 and cyclin-dependent kinase 4/6, thereby inducing PCa cell cycle arrest in G0/G1 phase and then inhibiting cell proliferation and clonogenicity. More important...Continue Reading

Citations

Mar 27, 2012·PloS One·Pierpaolo AimolaPier Paolo Claudio
Sep 28, 2014·Biochemical Pharmacology·Qing GongZhonghan Yang
Aug 6, 2004·Biochemical Pharmacology·Giuliana CassinelliFranco Zunino

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Methods Mentioned

BETA
transfection
protein assay
electrophoresis
Assay
transfecting
PCR

Software Mentioned

SPSS
CellQuest
RNAa

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