PMID: 16526280Mar 11, 2006Paper

Overexpression of p73 enhances cisplatin-induced apoptosis in HeLa cells

Archives of Pharmacal Research
Keun-Cheol KimKyung-Hee Choi

Abstract

To examine a possible synergistic role for p73 and cisplatin (cis-diamminedichloroplatinum II) in HeLa cells with a nonfunctional p53 protein, we established stable HeLa/p73 clones using a tetracycline inducible eukaryotic expression vector. The HeLa/p73 clones were not characterized by changes in growth or morphology. Cell death analysis, however, indicated a greater sensitivity to cisplatin in the p73-overexpressed HeLa cells than determined for the non-induced HeLa cells. This increased sensitivity seems to affect an induction of a sub-G1 population as assessed from flow cytometry analysis. The increased sub-G1 population may, in turn, result from a reduction of cyclin D1 and B1 expression by cisplatin in the presence of p73. Hoechest staining indicated an increased number of dead cells in the p73-induced cells compared to the non-induced cells. Poly ADP-ribose polymerase (PARP) cleavage was shown to be distinct in the p73-overexpressed cells compared to non-induced cells, which suggests that p73 modulates the cisplatin-induced apoptosis. Therefore, a synergistic effect of p73 and cisplatin to induce apoptosis could lead to new treatment for some types of human cancers.

References

May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T D KessisK R Cho
Jan 16, 1999·The Journal of Biological Chemistry·Z M YuanD Kufe
Aug 3, 1999·The Journal of Clinical Investigation·F BunzB Vogelstein
Jan 19, 2000·Cell Death and Differentiation·M LevreroJ Y Wang
Feb 9, 2000·Japanese Journal of Cancer Research : Gann·Y MinagawaN Terakawa
Jan 22, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lian DuanKiyotaka Yamamoto

❮ Previous
Next ❯

Citations

Jul 20, 2007·Blood·Arnon P KaterThomas J Kipps
Jul 21, 2007·Biochemical and Biophysical Research Communications·T M HansenG Melino
Oct 13, 2007·Molecular Carcinogenesis·M Fernanda LaraJesús M Paramio
May 6, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Allison WalterValérie B Schini-Kerth

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
T D KessisK R Cho
Cancer Cell
Meredith S IrwinWilliam G Kaelin
Cancer Biology & Therapy
Christopher E Barbieri, Jennifer A Pietenpol
© 2021 Meta ULC. All rights reserved