Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yufu TangWenping Zhou

Abstract

Gluconeogenesis, a reverse process of glycolysis, is suppressed in neoplastic livers. Cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C/PCK1, encoded by PCK1) is a step limiting enzyme of gluconeogenesis. The induced expression of the factor is reported to initiate gluconeogenesis process and antagonize hepatocellular carcinoma (HCC). In the current study, the effect of the modulation of PCK1 expression on HCC was assessed. The levels of PCK1 in clinical HCC tissues and different HCC cell lines were investigated with real time quantitative PCR, immunochemistry, and western blotting. Thereafter, the expression of PCK1 gene was induced in two HCC cell lines and the effect of the overexpression on proliferation and migration potentials of HCC cells was detected with CCK-8 assay, flow cytometry, TUNEL staining, and transwell assay. The activities of glycolysis and gluconeogenesis pathways in PCK1-overexpressed HCC cell lines were detected with specific kits to underlie the mechanism by which PCK1 exerted its function. The results of the in vitro experiments were validated with HCC xenograft rat models. The expression levels of PCK1 were suppressed in HCC samples and in cells derived from HCC tissues. According to the results o...Continue Reading

Citations

Apr 30, 2020·Experimental and Therapeutic Medicine·Yao LiuZhenqiang Qi
Dec 29, 2019·Journal of Molecular Recognition : JMR·Alaa M IsmailWael M Elshemey
Feb 6, 2019·Journal of Experimental & Clinical Cancer Research : CR·Lin TuoNi Tang
Aug 9, 2020·Cancer Management and Research·Huining TianGuixia Wang
Apr 6, 2019·Current Molecular Medicine·Venu SeenappaKapaettu Satyamoorthy
Feb 6, 2021·International Journal of Molecular Sciences·Gabriele GrasmannKatharina Leithner
Aug 31, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xu-Ping QianYong-Qing Wang

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