Overexpression of Peroxidase Gene GsPRX9 Confers Salt Tolerance in Soybean

International Journal of Molecular Sciences
Ting JinYan Li

Abstract

Peroxidases play prominent roles in antioxidant responses and stress tolerance in plants; however, their functions in soybean tolerance to salt stress remain unclear. Here, we investigated the role of a peroxidase gene from the wild soybean (Glycine soja), GsPRX9, in soybean tolerance to salt stress. GsPRX9 gene expression was induced by salt treatment in the roots of both salt-tolerant and -sensitive soybean varieties, and its relative expression level in the roots of salt-tolerant soybean varieties showed a significantly higher increase than in salt-sensitive varieties after NaCl treatment, suggesting its possible role in soybean response to salt stress. GsPRX9-overexpressing yeast (strains of INVSc1 and G19) grew better than the control under salt and H2O2 stress, and GsPRX9-overexpressing soybean composite plants showed higher shoot fresh weight and leaf relative water content than control plants after NaCl treatment. Moreover, the GsPRX9-overexpressing soybean hairy roots had higher root fresh weight, primary root length, activities of peroxidase and superoxide dismutase, and glutathione level, but lower H2O2 content than those in control roots under salt stress. These findings suggest that the overexpression of the GsPRX9...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Jianwei LiuChunzhao Zhao

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Methods Mentioned

BETA
transgenic
salt stress
PCR
electrophoresis
protein assay

Software Mentioned

Primer Premier
ClustalW2
GenScript
Search Tool for the Retrieval of Interacting Genes ( STRING
MEGA
SAS
Cytoscape

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