Overexpression of Peroxisome Proliferator-Activated Receptor γ Coactivator 1-α Protects Cardiomyocytes from Lipopolysaccharide-Induced Mitochondrial Damage and Apoptosis.

Inflammation
Tao ZhangWen-Liang Song

Abstract

Mitochondrial damage is considered one of the main pathogenetic mechanisms in septic cardiomyopathy. Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) is critical for maintaining energy homeostasis in different organs and in various physiological and pathological states. It is also a key regulator gene in mitochondrial metabolism. In this study, we investigated whether regulation of the PGC-1α gene had protective effects on septic cardiomyopathy. We developed a rat model of septic cardiomyopathy. H9c2 myocardiocytes were treated with lipopolysaccharide (LPS) and PGC-1α expression measured. PGC-1α-overexpressing lentivirus was used to transfect H9c2 cells. ZLN005 was used to activate PGC-1α. The effect of the inhibition of PGC-1α expression on myocardial cell injury and its underlying mechanisms were also explored. Cell viability was measured by CCK-8 assay. Mitochondrial damage was determined by measuring cellular ATP, reactive oxygen species, and the mitochondrial membrane potential. An apoptosis analysis kit was used to measure cellular apoptosis. Mitochondrial DNA was extracted and real-time PCR performed. LC3B, mitochondrial transcription factor A (TFA), P62, Bcl2, and Bax were determined by immunofluore...Continue Reading

References

Apr 24, 2008·Cardiovascular Research·Renée Ventura-ClapierVladimir Veksler
Jun 12, 2010·American Journal of Respiratory and Critical Care Medicine·Jane E CarréMervyn Singer
Jul 30, 2010·Journal of Inherited Metabolic Disease·Saskia Koene, Jan Smeitink
Oct 5, 2010·Circulation Research·Glenn C RoweZolt Arany
Jan 5, 2011·Antioxidants & Redox Signaling·Derek P Narendra, Richard J Youle
May 28, 2011·Science·Carmine SettembreAndrea Ballabio
Aug 30, 2011·Journal of Inherited Metabolic Disease·An I JonckheereRichard J T Rodenburg
Nov 4, 2011·Trends in Endocrinology and Metabolism : TEM·Ian S Patten, Zolt Arany
Oct 27, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·William T FestucciaYves Deshaies
Feb 6, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Marie-Hélène DisatnikDaria Mochly-Rosen
Mar 31, 2015·Cell Host & Microbe·Katie MaurerKen Cadwell
Aug 8, 2015·International Journal of Molecular Sciences·María Cecilia CimolaiHeiko Bugger
Feb 13, 2016·Shock·Nishkantha ArulkumaranUNKNOWN ADQI XIV Workgroup
May 14, 2016·Autophagy·Jeffery HoWilliam K K Wu
Sep 2, 2017·American Journal of Respiratory and Critical Care Medicine·Julie LavalDominik Hartl
Dec 15, 2017·Annals of Medicine·Dimitry A ChistiakovAlexander N Orekhov
Apr 12, 2018·The Journal of International Medical Research·Pan PanDawei Liu
Jun 2, 2018·Circulation·Yuxiao SunQun S Zang
Jun 4, 2018·Microvascular Research·Annette WegnerChristian Lehmann
Nov 8, 2018·International Journal of Molecular Sciences·Ching-Feng ChengHeng Lin
Sep 1, 2019·Molecular Therapy. Nucleic Acids·Tie-Ning ZhangChun-Feng Liu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
transfection
Assay
electrophoresis
PCR

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.