Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols

Nature Biotechnology
S R MuirM E Verhoeyen

Abstract

Tomatoes are an excellent source of the carotenoid lycopene, a compound that is thought to be protective against prostate cancer. They also contain small amounts of flavonoids in their peel ( approximately 5-10 mg/kg fresh weight), mainly naringenin chalcone and the flavonol rutin, a quercetin glycoside. Flavonols are very potent antioxidants, and an increasing body of epidemiological data suggests that high flavonoid intake is correlated with a decreased risk for cardiovascular disease. We have upregulated flavonol biosynthesis in the tomato in order to generate fruit with increased antioxidant capacity and a wider range of potential health benefit properties. This involved transformation of tomato with the Petunia chi-a gene encoding chalcone isomerase. Resulting transgenic tomato lines produced an increase of up to 78 fold in fruit peel flavonols, mainly due to an accumulation of rutin. No gross phenotypical differences were observed between high-flavonol transgenic and control lines. The phenotype segregated with the transgene and demonstrated a stable inheritance pattern over four subsequent generations tested thus far. Whole-fruit flavonol levels in the best of these lines are similar to those found in onions, a crop with...Continue Reading

References

Feb 27, 1995·Archives of Internal Medicine·M G HertogS Nedeljkovic
Feb 24, 1996·BMJ : British Medical Journal·P KnektJ Maatela
Mar 6, 1999·Current Opinion in Plant Biology·B Weisshaar, G I Jenkins
Sep 28, 1999·Trends in Plant Science·R A Dixon, C L Steele
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·I E Burbulis, B Winkel-Shirley
Jun 3, 2000·Nature Biotechnology·S RömerP M Bramley

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Citations

Sep 24, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M Cecilia RousseauxAnn Powell
Jul 19, 2005·Applied Microbiology and Biotechnology·Effendi LeonardMattheos A G Koffas
Apr 16, 2013·Plant Molecular Biology·Nigel E GapperJames J Giovannoni
Oct 31, 2006·Transgenic Research·Shuangyan ChenGongshe Liu
Jan 17, 2013·Applied Biochemistry and Biotechnology·Rajan Katoch, Neelam Thakur
Apr 15, 2004·Current Opinion in Biotechnology·Teresa Capell, Paul Christou
Dec 12, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C S Pérez-Coll, J Herkovits
Mar 9, 2004·Current Opinion in Plant Biology·Pierre Broun
Jun 1, 2005·Trends in Biotechnology·Toni M Kutchan
Feb 19, 2003·Phytochemistry·Lloyd W SumnerRichard A Dixon
Nov 22, 2002·Trends in Biotechnology·Ilya RaskinBertold Fridlender
Oct 18, 2001·Current Opinion in Biotechnology·I M van der MeerD Bosch
Apr 16, 2002·Current Opinion in Biotechnology·R Verpoorte, J Memelink
Apr 19, 2002·Current Opinion in Plant Biology·Brenda Winkel-Shirley
May 29, 2012·Journal of Agricultural and Food Chemistry·Pham Anh TuanSang Un Park
Sep 1, 2002·Molecular Plant Pathology·Richard A DixonLiangjiang Wang
May 11, 2002·The Proceedings of the Nutrition Society·I R Rowland
Nov 26, 2010·Critical Reviews in Food Science and Nutrition·Esra CapanogluRobert D Hall
Aug 30, 2008·Journal of Experimental Botany·Roosa A E LaitinenPaula Elomaa
Sep 27, 2005·Plant Physiology·Martine Lemaire-ChamleyChristophe Rothan
Aug 10, 2006·Plant Physiology·Sofia MocoC H Ric de Vos
Apr 24, 2007·Plant Physiology·Montserrat SaladiéJocelyn K C Rose
Sep 22, 2007·Plant Physiology·Yongzhen PangRichard A Dixon
Apr 29, 2008·Plant Physiology·Shira Mintz-OronAsaph Aharoni
Jul 10, 2008·Plant Physiology·Martina Newell-McGloughlin

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