Overexpression of phospholipase D suppresses taxotere-induced cell death in stomach cancer cells

Biochimica Et Biophysica Acta
Ju Hwan ChoJoong-Soo Han

Abstract

Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to generate phosphatidic acid (PA) and choline. There are at least two PLD isozymes, PLD1 and PLD2. Genetic and pharmacological approaches implicate both PLD isozymes in a diverse range of cellular processes, including receptor signaling, membrane transport control, and actin cytoskeleton reorganization. Several recent studies reported that PLD has a role in signaling pathways that oppose apoptosis and promote cell survival in cancer. In this study, we examined the role of PLD in taxotere-induced apoptosis in stomach cell lines; normal stomach (NSC) and stomach cancer cells (SNU 484). Taxotere treatment resulted in increase of PLD activity. To confirm the role of PLD in taxotere-induced apoptosis, PLDs were transfected into SNU 484 cells. Overexpression of PLD isozymes resulted in inhibition of taxotere-induced apoptotic cell death, evidenced by decreased degradation of chromosomal DNA, and increased cell viability. Concurrently, Bcl-2 expression was upregulated, and taxotere-induced activation of procaspase 3 was inhibited after PLD's transfection. However, when PLD was selectively inhibited by specific siRNA-PLD1 or -PLD2, taxotere-induced apoptosis was exa...Continue Reading

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Citations

Oct 8, 2011·Omics : a Journal of Integrative Biology·Xia Li, Ying-Jin Yuan
Mar 17, 2009·Experimental & Molecular Medicine·Hye-Jin ChoiJoong-Soo Han
Nov 29, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Maoxi LiuLi Zeng
Feb 27, 2008·Biochemical and Biophysical Research Communications·Michelle H WrightMatthew N Hodgkin
Oct 10, 2013·Advances in Biological Regulation·Julian Gomez-Cambronero
Feb 27, 2018·Journal of Molecular Neuroscience : MN·Shin-Young Park, Joong-Soo Han
Sep 8, 2019·Cellular and Molecular Neurobiology·Yun Young LeeJoong-Soo Han
Apr 30, 2016·Clinical Science·Junjie XiaoChangqing Yang
Sep 16, 2017·Scientific Reports·Tian LiuDavid E Kang

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