Overexpression of phytoene synthase gene from Salicornia europaea alters response to reactive oxygen species under salt stress in transgenic Arabidopsis

Biotechnology Letters
Heping HanShufeng Zhou

Abstract

A phytoene synthase gene SePSY was isolated from euhalophyte Salicornia europaea L. The 1655 bp full-length SePSY has an open reading frame of 1257 bp and encodes a 419-amino acid protein. The overexpression of SePSY enhanced the growth of transgenic Arabidopsis. When the plants were exposed to 100 mM NaCl, the photosynthesis rate and photosystem II activity (Fv/Fm) increased from 92% to 132% and from 9.3% to 16.6% in the transgenic lines than in the wild-type, respectively. The transgenics displayed higher activities of SOD and POD and lower contents of H(2)O(2) and MDA than the WT. In conclusion, the transgenic lines showed higher tolerance to salt stress than WT plants by increased photosynthesis efficiency and antioxidative capacity. This is the first report about improving the salt tolerance by genetic manipulation of carotenoid biosynthesis.

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Citations

May 15, 2012·The Arabidopsis Book·M Águila Ruiz-Sola, Manuel Rodríguez-Concepción
Feb 2, 2017·International Journal of Molecular Sciences·Yaogeng LeiPeiqiang Yu
Dec 24, 2019·Plant Signaling & Behavior·Cunxian Fan
Oct 18, 2019·BMC Plant Biology·Bliss Ursula FurtadoKatarzyna Hrynkiewicz
Nov 19, 2015·Frontiers in Plant Science·Yoshiki NakaharaHikaru Sakamoto
Jul 1, 2016·Functional Plant Biology : FPB·Ceyda Ozfidan-KonakciIsmail Turkan
Dec 2, 2020·The Plant Journal : for Cell and Molecular Biology·Juan C MorenoSalim Al-Babili
Jan 24, 2021·Journal of Experimental Botany·Stella KösslerJuan C Moreno

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