Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein

International Journal of Molecular Medicine
Linna ZhangQikuan Hu

Abstract

PR (PRDI‑BFI and RIZ) domain containing (PRDM) proteins have been shown to be important in several types of human cancer. PRDM13, a member of the PRDM family, contains transcriptional regulators involved in modulating several cellular processes. However, the function of PRDM13 in glioma remains to be elucidated. The purpose of the present study was to evaluate the expression and effect of PRDM13 on glioma cells. It was found that the expression of PRDM13 was reduced in glioma cells, and the overexpression of PRDM13 significantly decreased the proliferation, migration and invasion of U87 glioma cells. Through validation of RNA‑sequencing analysis, genes regulating cell proliferation and migration were classified from Gene Ontology sources. In addition, PRDM13 was shown to be associated with Rho protein and GTP enzyme activation protein. The over-expression of PRDM13 upregulated deleted in liver cancer 1 (DLC1) to inhibit the proliferation and invasion of U87 cells. In conclusion, PRDM13 decreased the proliferation and invasion of U87 cells, and may be of potential value for glioma therapy.

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Citations

Apr 16, 2020·International Journal of Molecular Sciences·Amelia CasamassimiCiro Abbondanza

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Methods Mentioned

BETA
fluorescence microscopy
PCR
GTPase
Infrared Imaging
transfection
light microscopy
RNA-Seq
immunoprecipitation
ChIP
flow cytometry

Software Mentioned

Basic Local Alignment Search Tool
Image Studio
SPSS

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