Overexpression of protein kinase C ɛ improves retention and survival of transplanted mesenchymal stem cells in rat acute myocardial infarction

Cell Death & Disease
H HeY-J Zeng

Abstract

We assessed the effects of protein kinase C ɛ (PKCɛ) for improving stem cell therapy for acute myocardial infarction (AMI). Primary mesenchymal stem cells (MSCs) were harvested from rat bone marrow. PKCɛ-overexpressed MSCs and control MSCs were transplanted into infarct border zones in a rat AMI model. MSCs and PKCɛ distribution and expression of principal proteins involved in PKCɛ signaling through the stromal cell-derived factor 1 (SDF-1)/CXC chemokine receptor type 4 (CXCR4) axis and the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway were analyzed by immunofluorescence and western blot 1 day after transplantation. Echocardiographic measurements and histologic studies were performed at 4 weeks after transplantation, and MSC survival, expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), transforming growth factor β (TGFβ), cardiac troponin I (cTnI), von Willebrand factor (vWF), smooth muscle actin (SMA) and factor VIII and apoptosis in infarct border zones were assessed. Rat heart muscles retained more MSCs and SDF-1, CXCR4, PI3K and phosphorylated AKT increased with PKCɛ overexpression 1 day after transplantation. MSC survival and VEGF, bFGF, TGFβ, cTnI, vWF, SMA...Continue Reading

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Citations

Mar 15, 2018·Stem Cells International·Heiko LemckeRobert David
Jan 10, 2019·Stem Cells International·Zhi ChenWei Eric Wang
Feb 1, 2020·Journal of Translational Medicine·Dickson Kofi Wiredu OcanseyFei Mao
Oct 8, 2020·International Journal of Molecular Sciences·Kamila RaziyevaArman Saparov
Feb 13, 2021·Frontiers in Cardiovascular Medicine·Weili ShiKeji Chen
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ruixi LuoYounan Chen
Jun 9, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Li ChenMing Guo

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Methods Mentioned

BETA
transfection
SMA
flow cytometry
PCR
reverse transcription-PCR

Software Mentioned

ImageQuant TL
Image
Pro Plus

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