Overexpression of Pyrabactin Resistance-Like Abscisic Acid Receptors Enhances Drought, Osmotic, and Cold Tolerance in Transgenic Poplars

Frontiers in Plant Science
Jingling YuLei Yang

Abstract

Abscisic acid (ABA) has been known participate in a wider range of adaptive responses to diverse environmental abiotic stresses such as drought, osmosis, and low temperatures. ABA signaling is initiated by its receptors PYR/PYL/RCARs, a type of soluble proteins with a conserved START domain which can bind ABA and trigger the downstream pathway. Previously, we discovered that poplar (Populus trichocarpa) genome encodes 14 PYR/PYL/RCAR orthologs (PtPYRLs), and two of them, PtPYRL1 and PtPYRL5 have been functionally characterized to positively regulate drought tolerance. However, the physiological function of these ABA receptors in poplar remains uncharacterized. Here, we generated transgenic poplar plants overexpressing PtPYRL1 and PtPYRL5 and found that they exhibited more vigorous growth and produced greater biomass when exposed to drought stress. The improved drought tolerance was positively correlated with the key physiological responses dictated by the ABA signaling pathway, including increase in stomatal closure and decrease in leaf water loss. Further analyses revealed that overexpression lines showed improved capacity in scavenging reactive oxygen species and enhanced the activation of antioxidant enzymes under drought st...Continue Reading

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Citations

Jul 10, 2018·Plant & Cell Physiology·Wim DejongheSean R Cutler
Nov 27, 2019·Journal of Experimental Botany·Maximiliano Estravis-BarcalaMaría Verónica Arana
Jan 24, 2019·Frontiers in Plant Science·Andrea PolleAntoine Harfouche
Jun 6, 2019·BMC Genomics·Camila Campos MantelloAnete Pereira de Souza
May 6, 2019·The New Phytologist·Dade YuAndrea Polle
Nov 22, 2019·Frontiers in Plant Science·Zihan ChengTingbo Jiang
Dec 19, 2019·Frontiers in Plant Science·Rakesh Kumar VermaViswanathan Chinnusamy
Jan 26, 2021·Frontiers in Plant Science·Tao YaoJin-Gui Chen
Oct 26, 2021·The Plant Journal : for Cell and Molecular Biology·Rubén Tenorio BerríoMarieke Dubois

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Methods Mentioned

BETA
transgenic
PCR
Assay
MDA

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