Overexpression of Pyruvate Kinase Type M2 (PKM2) Promotes Ovarian Cancer Cell Growth and Survival Via Regulation of Cell Cycle Progression Related with Upregulated CCND1 and Downregulated CDKN1A Expression

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Bin ZhengQiaojia Huang

Abstract

BACKGROUND Many findings have shown that pyruvate kinase type M2 (PKM2) plays crucial roles in regulating the occurrence and development of various human cancers; however, its roles in ovarian cancer oncogenesis remain to be determined. MATERIAL AND METHODS The expression intensity of PKM2 in ovarian cancer tissues was examined by immunohistochemistry (IHC), and was then correlated to patient clinicopathologic characteristics. The roles of PKM2 in ovarian cancer cell proliferation, growth, and survival were examined by CCK-8, colony forming, and flow cytometry assays. The potentially involved molecular were then investigated by Western blot analysis. RESULTS IHC results showed that PKM2 was overexpressed in 100 of 114 (87.7%) serous ovarian cancer tissues as compared with 50 cases of non-cancerous ovarian tissues, and was associated with tumor size ≥7.5 cm and <7.5 cm (p<0.05). Overexpression of PKM2 in SKOV3 and HEY ovarian cancer cells by transfection with PKM2 lentivirus vector led to increased cell proliferation, growth, and survival, which may be related with PKM2 being able to increase cell cycle progress: G1 stage decreased, whereas S stage significantly increased. In contrast, all functions of SKOV3 and HEY cells descri...Continue Reading

Citations

Feb 6, 2020·Mass Spectrometry Reviews·Na Li, Xianquan Zhan
Feb 29, 2020·Journal of Hepatocellular Carcinoma·Tong LiuChuanyong Guo
Dec 4, 2019·Oncology Letters·Qiongli SuXiaoping Yang
Apr 10, 2021·Frontiers in Cell and Developmental Biology·Thais SobanskiEmma Bolderson

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surgical resection
PCR
flow cytometry

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