Overexpression of RCN1 correlates with poor prognosis and progression in non-small cell lung cancer

Human Pathology
Xinming ChenHonggang Ke

Abstract

We investigated the expression of reticulocalbin-1 (RCN1) and its prognostic significance in non-small cell lung cancer (NSCLC). RCN1 expression was evaluated by immunohistochemical analysis with tissue microarrays in NSCLC tissues and matched adjacent noncancerous tissues. Furthermore, quantitative polymerase chain reaction and Western blot were also used to examine the expression of RCN1. Moreover, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, clone formation, and transwell assays were used to measure cell proliferation, migration, and invasion. Lastly, we used the Kaplan-Meier method and log-rank test to compare overall survival rates between the RCN1-high expression group and the RCN1-low expression group. In this study, immunohistochemistry by tissue microarray at RCN1 expression was significantly up-regulated in NSCLC tissues compared with adjacent noncancerous tissues. We further confirmed the up-regulation of RCN1 by quantitative polymerase chain reaction and Western blot assay. RCN1 expression level was closely related to lymph node metastasis (P < .001) and TNM stage (P = .012). Kaplan-Meier analysis showed that high RCN1 expression was remarkably associated with poor prognosis with NSCLC patients...Continue Reading

References

Aug 12, 2003·Cardiovascular Research·Rafal GrzeskowiakPatricia Ruiz
May 19, 2005·International Journal of Cancer. Journal International Du Cancer·Takashi HiranoNorihiko Ikeda
Jan 21, 2011·The Lancet Oncology·Chihua Wen, Tim Dehnel
Jan 29, 2011·Biochemical and Biophysical Research Communications·Yasunobu YoshidaShin-ichi Tsunoda
Aug 7, 2013·Journal of Proteomics·Giuliana GiribaldiPaolo Destefanis
Dec 5, 2013·TheScientificWorldJournal·Sayf GazalaEric L R Bédard
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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