Overexpression of regucalcin suppresses cell death and apoptosis in cloned rat hepatoma H4-II-E cells induced by lipopolysaccharide, PD 98059, dibucaine, or Bay K 8644

Journal of Cellular Biochemistry
Takako Izumi, Masayoshi Yamaguchi

Abstract

The effect of regucalcin, a regulatory protein in intracellular signaling pathway, on cell death was investigated by using the cloned rat hepatoma H4-II-E cells overexpressing regucalcin. The hepatoma cells (wild-type) and stable regucalcin (RC)/pCXN2 transfectants were cultured for 72 h in medium containing 10% fetal bovine serum (FBS) to obtain subconfluent monolayers. After culture for 72 h, cells were further cultured for 12-72 h in medium without FBS containing either vehicle or lipopolysaccharide (LPS; 0.1 or 1.0 microg/ml). The number of wild-type cells was significantly decreased by culture for 24 or 48 h in the presence of LPS (0.1 or 1.0 microg/ml). The effect of LPS (0.1 or 1.0 microg/ml) in decreasing the number of hepatoma cells was significantly prevented in transfectants overexpressing regucalcin. However, the culture with LPS (0.1 or 1.0 microg/ml) for 72 h caused a significant decrease in cell number of transfectants. Ca(2+)/calmodulin-dependent nitric oxide (NO) synthase activity was significantly decreased by culture with LPS (1.0 microg/ml) for 24-72 h of wild-type cells. This decrease was significantly prevented in transfectants. LPS (0.1 or 1.0 microg/ml)-induced decrease in the number of wild-type cells w...Continue Reading

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Citations

May 15, 2013·Apoptosis : an International Journal on Programmed Cell Death·Masayoshi Yamaguchi
May 18, 2010·Obesity Surgery·Samer GawriehMichael Olivier
Nov 29, 2011·Molecular Human Reproduction·Sandra S LaurentinoSílvia Socorro
Jun 18, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·S S LaurentinoS Socorro
Mar 23, 2013·Cellular and Molecular Life Sciences : CMLS·Ricardo MarquesSílvia Socorro
Mar 13, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yoon-Ha KimChan-Wha Kim
Apr 15, 2016·International Journal of Molecular Sciences·Shinpei KadowakiIsao Kubota
Mar 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jin TaoShengnan Li
Oct 31, 2006·World Journal of Gastroenterology : WJG·Yoshiyuki TakaharaNorifumi Kawada
Jul 31, 2012·Biomolecular Concepts·Stephanie H Scott, Brian J Bahnson
Jul 30, 2005·Journal of Cellular Biochemistry·Masayoshi YamaguchiSatoshi Uchiyama
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi

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