Overexpression of Rosea1 From Snapdragon Enhances Anthocyanin Accumulation and Abiotic Stress Tolerance in Transgenic Tobacco

Frontiers in Plant Science
Aung Htay NaingChang K Kim

Abstract

The co-expression of Rosea1 (Ros1) and Delila (Del) regulates anthocyanin levels in snapdragon flowers, as well as in tomato, petunia, and tobacco. However, there is little information on how Ros1 expression alone controls anthocyanin regulation and whether it is involved in the mechanism that leads to abiotic stress tolerance. In the present study, tobacco (Nicotiana tabacum 'Xanthi') transgenic plants overexpressing Ros1 (T2-Ros1-1, T2-Ros1-2, T2-Ros1-3, and T2-Ros1-4) promoted accumulation of anthocyanin in leaves and flowers by elevating the transcription of all key genes involved in the biosynthesis of this pigment. This promotion largely occurred through the upregulation of dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase genes in leaves and upregulation of DFR in flowers. Under normal conditions, the transgenic lines and wild type (WT) plants showed well-developed broad leaves and regular roots, whereas a reduction in plant growth was observed under cold and drought stresses. However, the transgenic T2-Ros1 lines were able to tolerate the stresses better than the WT line by inducing reactive oxygen species scavenging activities, and the expression of antioxidant-related and stress-responsive genes. In additi...Continue Reading

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Citations

Apr 17, 2020·Photochemistry and Photobiology·Nahuel I CubaM Gabriela Lagorio
Feb 20, 2021·Physiologia Plantarum·Aung Htay Naing, Chang Kil Kim
May 18, 2021·Frontiers in Plant Science·Yanfei ZhouKathy E Schwinn

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Methods Mentioned

BETA
transgenic
phosphotransferase
PCR
MDA

Software Mentioned

MEGA7
SPSS
BioNJ

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