Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.

Journal of Experimental Botany
Linda M TabeRuediger Hell

Abstract

There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosynthesis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F(2) seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur am...Continue Reading

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Citations

Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Anna WawrzyńskaAgnieszka Sirko
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Sumita Acharjee, Bidyut Kumar Sarmah
Sep 2, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Anna Koprivova, Stanislav Kopriva
Feb 16, 2017·Frontiers in Plant Science·Karen M FrickRhonda C Foley
Apr 4, 2013·International Journal of Molecular Sciences·Lyuben ZagorchevMariela Odjakova
Jan 23, 2016·Evolutionary Bioinformatics Online·Luca AmbrosinoMaria L Chiusano
Mar 3, 2019·Journal of Experimental Botany·Joseph M Jez
Jun 20, 2019·The Plant Journal : for Cell and Molecular Biology·Jaya JoshiFrédéric Marsolais
Mar 27, 2019·Journal of Experimental Botany·Rachel AmirYael Hacham
Jan 13, 2019·Plants·Angelo De PaolisSofia Caretto
May 10, 2018·Frontiers in Plant Science·Nicola M Capstaff, Anthony J Miller
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder

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Datasets Mentioned

BETA
CA410528
EF455724

Methods Mentioned

BETA
transgenic
PCR

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