Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance

FEMS Microbiology Letters
Rungaroon Waditee-SirisatthaTeruhiro Takabe

Abstract

Serine hydroxymethyltransferase (SHMT) is a key enzyme in cellular one-carbon pathway and has been studied in many living organisms from bacteria to higher plants and mammals. However, biochemical and molecular characterization of SHMT from photoautotrophic microorganisms remains a challenge. Here, we isolated the SHMT gene from a halotolerant cyanobacterium Aphanothece halophytica (ApSHMT) and expressed it in Escherichia coli. Purified recombinant ApSHMT protein exhibited catalytic reactions for dl-threo-3-phenylserine as well as for l-serine. Catalytic reaction for l-serine was strongly inhibited by NaCl, but not to that level with glycine betaine. Overexpression of ApSHMT in E. coli resulted in the increased accumulation of glycine and serine. Choline and glycine betaine levels were also significantly increased. Under high salinity, the growth rate of ApSHMT-expressing cells was faster compared to its respective control. High salinity also strongly induced the transcript level of ApSHMT in A. halophytica. Our results indicate the importance of a novel pathway; salt-induced ApSHMT increased the level of glycine betaine via serine and choline and conferred the tolerance to salinity stress.

References

Jul 1, 1985·Journal of Bacteriology·V SchirchS Angelaccio
Feb 1, 1993·Journal of Bacteriology·R L WilsonG V Stauffer
Jul 24, 1998·Protein Expression and Purification·M L di SalvoV Schirch
Jan 22, 2002·Metabolic Engineering·Denis RonteinAndrew D Hanson
Apr 19, 2002·Current Opinion in Plant Biology·Tony H H Chen, Norio Murata
Aug 23, 2002·The Journal of Biological Chemistry·Takashi HibinoTeruhiro Takabe
Aug 9, 2005·Applied and Environmental Microbiology·Haruo MisonoShinji Nagata
Dec 13, 2005·Plant Physiology·Lars M VollAndreas P M Weber
Dec 5, 2006·Applied and Environmental Microbiology·Michael StolzLothar Eggeling
Sep 22, 2007·The Journal of Biological Chemistry·Rungaroon WaditeeTeruhiro Takabe
Jan 10, 2008·Journal of Experimental Botany·Nazmul H BhuiyanTeruhiro Takabe
Jun 11, 2009·PloS One·Donald D Anderson, Patrick J Stover
Apr 21, 2010·Trends in Plant Science·Hermann BauweAlisdair R Fernie
Dec 18, 2010·Microbiology·Ashish Kumar SrivastavaBrett A Neilan
Feb 25, 2011·The American Journal of Clinical Nutrition·Anna E BeaudinPatrick J Stover

❮ Previous
Next ❯

Citations

Dec 29, 2012·PloS One·Simone Schneider WeberCélia Maria de Almeida Soares
Nov 18, 2014·Journal of Fish Diseases·C M J DelannoyM C Fontaine
May 29, 2016·Applied and Environmental Microbiology·Song HuangGwénaël Jan
Jul 2, 2021·Advanced Science·Junho BangSang Yup Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.