Overexpression of sigma factor, sigma(B), urges Staphylococcus aureus to thicken the cell wall and to resist beta-lactams

Biochemical and Biophysical Research Communications
K MorikawaT Ohta

Abstract

Whole genome sequence analysis revealed that Staphylococcus aureus is provided with only a few sigma factors, including the alternative sigma factor, sigma(B), which is thought to regulate some stress responses. Since the sigB knock-out mutant did not show remarkable phenotypic difference, we constructed the over expressed mutant to examine the role of the sigB. Electron microscopic observation revealed that the mutant showed a variety of cell sizes compared with the parent strain which showed almost homogeneous cell sizes. The mutant delivered a thicker cell wall, about 20% thicker than the parent strain. It became resistant to the lytic activity of lysostaphin and also raised MICs to the cell-wall-affecting antibiotics. The yield of carotenoids and transcripts of pbps were also increased in the mutant. The result suggests that sigB plays some important roles in cell wall synthesis and in resistance to antibiotics that perturb the cell wall synthesis.

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Citations

Jan 20, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Kazuya MorikawaToshiko Ohta
May 24, 2012·The Journal of Antimicrobial Chemotherapy·Keith Poole
Sep 15, 2010·Antimicrobial Agents and Chemotherapy·Longzhu CuiKeiichi Hiramatsu
Jun 2, 2011·Antimicrobial Agents and Chemotherapy·Mitsutaka ShojiKeiichi Hiramatsu
Mar 27, 2012·Applied and Environmental Microbiology·Avelino Alvarez-OrdóñezColin Hill
Dec 24, 2011·Journal of Bacteriology·Jakob EngmanClaes von Wachenfeldt
Nov 18, 2005·Journal of Bacteriology·Samuel KatzifWilliam M Shafer
Nov 24, 2007·Annual Review of Microbiology·Michael HeckerUwe Völker
Jan 26, 2012·BMC Microbiology·Bettina SchulthessBrigitte Berger-Bächi
Feb 21, 2007·The Journal of General and Applied Microbiology·Yumiko InoseToshiko Ohta
Jul 29, 2005·Antimicrobial Agents and Chemotherapy·Longzhu CuiKeiichi Hiramatsu
Apr 19, 2010·Pharmaceuticals·Maria do Carmo de Freire BastosMarcus Lívio Varella Coelho
Jan 13, 2009·Journal of Theoretical Biology·Elsje PienaarNicolaas F J van Rensburg
Mar 26, 2016·Frontiers in Microbiology·Maria BartolomeuAdelaide Almeida
May 13, 2015·International Journal of Systematic and Evolutionary Microbiology·Stefanie Heß, Claudia Gallert
Jul 24, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya MorikawaToshiko Ohta
Apr 14, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya MorikawaKunio Takeyasu
Jan 24, 2015·Antonie van Leeuwenhoek·Khadar Syed Musthafa, Supayang Piyawan Voravuthikunchai
Apr 29, 2006·International Journal of Medical Microbiology : IJMM·Jan Pané-FarréMichael Hecker
Sep 22, 2016·The Journal of Antimicrobial Chemotherapy·Jessica Z Kubicek-SutherlandDan I Andersson
May 22, 2016·Applied and Environmental Microbiology·Claudia GuldimannVeronica Guariglia-Oropeza
Aug 27, 2014·Antimicrobial Agents and Chemotherapy·Dhritiman Samanta, Mohamed O Elasri
Jul 28, 2016·Antimicrobial Agents and Chemotherapy·Katrin SchilcherAnnelies S Zinkernagel

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