Overexpression of signal transducers and activators of transcription in embryos derived from vitrified oocytes negatively affect E-cadherin expression and embryo development

Cryobiology
Abolfazl ShiraziZahra Derafshian

Abstract

Vitrification apart from all drawbacks on oocyte ultra-structure can affect the oocyte mRNA content. Among those evaluated transcripts, no data is available regarding the effect of vitrification on signal transducer and activator of transcription (STAT3) expression in oocytes and the resulting preimplantation embryos. Considering the bidirectional relationship between E-cadherin (CDH1) and STAT3 and the adverse effect of cryopreservation on adherent junctions, we aimed to ascertain to what extent STAT3 and CDH1 genes expression is affected by vitrification in oocytes and the resulting embryos. The ovine vitrified-warmed and fresh GV oocytes were separately subjected to in vitro maturation and fertilization and cultured up to the blastocyst stage. The relative abundance of STAT3 and CDH1 transcripts were analysed by RT-PCR in both classes of fresh and vitrified GV and MII oocytes and the resulting embryos at 2-7 cells, 8-16 cells, morula, and blastocyst stages. Vitrified oocytes showed lower cleavage (37.8% vs. 95.9%, P<0.001) and blastocyst (8.1% vs. 52.7%, P<0.001) rates compared to control. The relative mRNA abundance of both genes was increased after oocyte maturation indicating their expression was started earlier than expe...Continue Reading

References

Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LarueR Kemler
Oct 1, 1996·Current Opinion in Cell Biology·O HuberR Kemler
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·K TakedaS Akira
Sep 12, 1997·Science·J E Darnell
Jun 13, 2000·Oncogene·T BowmanR Jove
Dec 31, 2002·Molecular Reproduction and Development·H MenJ J Rutledge
Apr 22, 2003·Molecular Reproduction and Development·Rui-Hua LiuWei-Hua Wang
May 20, 2003·Human Reproduction Update·C A AmorimJ R Figueiredo
Dec 25, 2003·Molecular Reproduction and Development·Trudee FairPatrick Lonergan
Jul 24, 2004·Animal Reproduction Science·A J WatsonL C Barcroft
Jul 28, 2004·Molecular Reproduction and Development·Chun-Bo TengZeng-Ming Yang
Jul 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Chun-Bo TengZeng-Ming Yang
Sep 24, 2005·Reproductive Biomedicine Online·Masashige KuwayamaStanley P Leibo
Mar 17, 2006·Molecular Reproduction and Development·Duangjai BoonkusolAndras Dinnyes
Jun 8, 2006·Fertility and Sterility·Yehudith GhetlerRuth Shalgi
Aug 31, 2006·Molecular Reproduction and Development·G G LeoniS Naitana
Mar 29, 2007·Reproduction, Fertility, and Development·Sergio LeddaSalvatore Naitana
Sep 22, 2007·Molecular Reproduction and Development·Sara SuccuSergio Ledda
Nov 12, 2009·Journal of Assisted Reproduction and Genetics·Vahida M AnchamparuthyFrancis C Gwazdauskas
Jan 6, 2010·Reproduction, Fertility, and Development·Silvia ModinaAlberto Maria Luciano
Dec 22, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wolfgang GlienkeLothar Bergmann
Jul 16, 2014·The International Journal of Developmental Biology·Alison M HantakMilan K Bagchi

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