Overexpression of SmMYB9b enhances tanshinone concentration in Salvia miltiorrhiza hairy roots

Plant Cell Reports
Jingxian ZhangShujuan Zhao

Abstract

A Salvia miltiorrhiza R2R3-MYB gene, SmMYB9b , has been cloned and characterized. Overexpression of SmMYB9b resulted in a significant improvement of tanshinones, the lipophilic active ingredients in danshen hairy roots. Plant R2R3-MYB transcription factors play important roles in various physiological and biochemical processes. Danshen (Salvia miltiorrhiza bunge) is a valuable medicinal herb with tanshinones and salvianolic acids as the principal bioactive ingredients. A number of putative R2R3-MYB transcription factors have been identified in the plant, but their function remains to be studied. Here, we report the cloning of SmMYB9b, an S20 R2R3-MYB member and its regulatory properties. SmMYB9b contains an open reading frame of 792 bp in length and encodes a 264-amino acid protein. Its transcripts were most abundant in blooming flowers (except for calyces) and increased with flower development. Exogenous abscisic acid strongly activated its transcription. Gibberellins and methyl jasmonate also showed a time-dependent activation effect on its transcription, but to a weaker degree. Overexpression of SmMYB9b in danshen hairy roots enhanced tanshinone concentration to 2.16 ± 0.39 mg/g DW, a 2.2-fold improvement over the control. I...Continue Reading

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Citations

Apr 29, 2020·Journal of Integrative Plant Biology·Changping DengGuoyin Kai
Oct 24, 2018·Plant Science : an International Journal of Experimental Plant Biology·Bingcong XingZongsuo Liang
Apr 4, 2021·International Journal of Molecular Sciences·Xiuhong LiJuane Dong

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Datasets Mentioned

BETA
JX113685
JX113686
KF059363

Methods Mentioned

BETA
transgenic
PCR
RNAseq

Software Mentioned

Primer Premier
BLASTX
MegAlign
MEGA
Finnigan Xcalibur
SPSS19

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