Overexpression of SSBXoc , a Single-Stranded DNA-Binding Protein From Xanthomonas oryzae pv. oryzicola , Enhances Plant Growth and Disease and Salt Stress Tolerance in Transgenic Nicotiana benthamiana

Frontiers in Plant Science
Yanyan CaoGong-you Chen

Abstract

We previously reported that SSBXoc, a highly conserved single-stranded DNA-binding protein from Xanthomonas spp., was secreted through the type III secretion system (T3SS) and functioned as a harpin-like protein to elicit the hypersensitive response (HR) in the non-host plant, tobacco. In this study, we cloned SsbXoc gene from X. oryzae pv. oryzicola (Xoc), the causal agent of bacterial leaf streak in rice, and transferred it into Nicotiana benthamiana via Agrobacterium-mediated transformation. The expression of SsbXoc in transgenic N. benthamiana enhanced growth of both seedling and adult plants. When inoculated with the harpin Hpa1 or the pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), the accumulation of reactive oxygen species (ROS) was increased more in SsbXoc transgenic lines than that in wild-type (WT) plants. The expression of pathogenesis-related protein genes (PR1a and SGT1), HR marker genes (HIN1 and HSR203J) and the mitogen-activated protein kinase pathway gene, MPK3, was significantly higher in transgenic lines than in WT after inoculation with Pst DC3000. In addition, SsbXoc transgenic lines showed the enhanced resistance to the pathogenic bacteria P. s. tabaci and the improved tolerance to salt stre...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Feb 3, 1999·Annual Review of Genetics·P R Johnson, J R Ecker
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Jun 5, 2002·The Plant Cell·Kevin L-C WangJoseph R Ecker
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Kazuya YoshimuraShigeru Shigeoka
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Feb 22, 2005·Brain Research. Brain Research Protocols·Martin Alexander Thomas, Björn Lemmer
Apr 16, 2005·Current Opinion in Biotechnology·Basia Vinocur, Arie Altman
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Marcus A SamuelBrian E Ellis
Jun 15, 2005·Trends in Plant Science·Hirofumi NakagamiHeribert Hirt
Feb 24, 2006·Current Opinion in Biotechnology·Taishi UmezawaKazuo Shinozaki
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Sep 15, 2007·Trends in Plant Science·Viswanathan ChinnusamyJian-Kang Zhu
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Dec 17, 2009·Physiologia Plantarum·Miguel Angel Torres
Apr 10, 2010·Journal of Integrative Plant Biology·Amrit K NandaChristophe Dunand
Apr 13, 2010·Plant Signaling & Behavior·Matias D ZurbriggenMohammad-Reza Hajirezaei
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Takashi Hirayama, Kazuo Shinozaki
Aug 7, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Arzu Tezvergil-MutluayDavid H Pashley
Feb 1, 2011·The Plant Cell·Bart P H J ThommaMatthieu H A J Joosten
Apr 30, 2011·Journal of Experimental Botany·Lei ZhangMin Shao
Jun 12, 2013·Molecular Plant-microbe Interactions : MPMI·Min-Seon ChoiChang-Sik Oh

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
MDA
environmental stress

Software Mentioned

SPSS
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.