Overexpression of TFF3 is involved in prostate carcinogenesis via blocking mitochondria-mediated apoptosis.

Experimental & Molecular Medicine
Jieying LiuYeun-Jun Chung

Abstract

The overexpression of trefoil factor family 3 (TFF3) is observed in a variety of cancers, including prostate cancer (PCa), and its potential role in carcinogenesis, such as activating the PI3K/AKT pathway, is suggested. However, its role and its related mechanisms in prostate tumorigenesis remain unknown. To elucidate the role of TFF3 overexpression in PCa, we silenced TFF3 in two PCa cell lines that overexpressed TFF3 and explored the molecular mechanism behind its antiapoptotic role. We also examined TFF3 expression in 108 Korean PCa specimens and 106 normal prostate tissues by immunohistochemistry (IHC) analysis. The mean TFF3 IHC score in the tumor tissues was significantly higher than that in the normal tissues (4.702 vs. 0.311, P = 2.52 × 10-24). TFF3-silenced cells showed suppressed tumor cell growth and migration. TFF3 silencing decreased BCL2 and increased BAX expression. The translocation of BAX to the mitochondria was also confirmed. After TFF3 silencing, the expression of the mitochondrial proapoptotic proteins, cytochrome C and Smac/DIABLO, was elevated, and these proteins were released from the mitochondria to the cytosol. Downstream mediators of mitochondrial apoptosis, including cleaved caspase-3, caspase-9, and...Continue Reading

References

Mar 1, 1992·The Journal of Urology·F LabrieJ Emond
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·D R TaupinD K Podolsky
Feb 7, 2001·The Journal of Biological Chemistry·S PugazhenthiJ E Reusch
Aug 5, 2000·Biochemical and Biophysical Research Communications·Y H ChenX D Tan
Apr 20, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·D TaupinA S Giraud
May 10, 2002·The American Journal of Pathology·Benoit TerrisNicholas R Lemoine
May 11, 2002·Nature Reviews. Cancer·Frederik H Igney, Peter H Krammer
Sep 5, 2002·Nature Reviews. Cancer·Suzanne Cory, Jerry M Adams
Feb 26, 2003·Cell·Donald D Newmeyer, Shelagh Ferguson-Miller
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Sep 16, 2004·The Prostate·Isla P GarrawayRobert E Reiter
Jun 7, 2005·Cell·Douglas R Green
Oct 29, 2005·Science·Scott A TomlinsArul M Chinnaiyan
Dec 24, 2005·Cellular and Molecular Life Sciences : CMLS·W Hoffmann
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Else Marie VestergaardNiels Tørring
Jan 24, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jens MadsenUffe Holmskov
May 5, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ashley E RossPatrick C Walsh
Jan 17, 2012·The Journal of Urology·Maciej Salagierski, Jack A Schalken
Apr 21, 2012·International Journal of Radiation Oncology, Biology, Physics·Enrique CasadoPaloma Cejas
May 23, 2012·Nature Genetics·Christopher E BarbieriLevi A Garraway
Jun 23, 2012·Nature·Catherine S GrassoScott A Tomlins
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Oct 16, 2013·Journal of Cancer Research and Therapeutics·You-Guang HuangYun Zhang
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Mar 20, 2014·JAMA : the Journal of the American Medical Association·Julia H Hayes, Michael J Barry
May 30, 2014·The Journal of Pathology·Tae-Min KimSug-Hyung Lee
Apr 23, 2015·Endocrine-related Cancer·Felicity E B May, Bruce R Westley
Nov 7, 2015·European Urology·Seung-Hyun JungYeun-Jun Chung
Jan 19, 2016·Investigative Ophthalmology & Visual Science·Julia DieckowFriedrich P Paulsen
Nov 24, 2016·Oncotarget·Martina KluthRonald Simon
Dec 30, 2016·Future Science OA·Pranav SharmaJulio M Pow-Sang

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
reverse transcription-PCR
Assay
biopsy
FACS

Software Mentioned

SPSS
ImageJ

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.