Overexpression of TGFβ1 in murine mesenchymal stem cells improves lung inflammation by impacting the Th17/Treg balance in LPS-induced ARDS mice.

Stem Cell Research & Therapy
Jianxiao ChenHaibo Qiu

Abstract

T helper 17 cells (Th17)/regulatory T cells (Treg), as subtypes of CD4+ T cells, play an important role in the inflammatory response of acute respiratory distress syndrome (ARDS). However, there is still a lack of effective methods to regulate the differentiation balance of Th17/Treg. It was proven that mesenchymal stem cells (MSCs) could regulate the differentiation of CD4+ T cells, but the mechanism is still unclear. TGFβ1, a paracrine cytokine of MSCs, could also regulate the differentiation of Th17/Treg but is lowly expressed in MSCs. Therefore, mouse MSCs (mMSCs) overexpressing TGFβ1 were constructed by lentivirus transduction and intratracheally transplanted into LPS-induced ARDS mice in our study. The aim of this study was to evaluate the therapeutic effects of mMSCs overexpressing TGFβ1 on inflammation and immunoregulation by impacting the Th17/Treg balance in LPS-induced ARDS mice. mMSCs overexpressing TGFβ1 were constructed using lentiviral vectors. Then, mouse bone-marrow-derived MSCs (mBM-MSC) and mBM-MSC-TGFβ1 (mBM-MSC overexpressing TGFβ1) were transplanted intratracheally into ARDS mice induced by lipopolysaccharide. At 3 and 7 days after transplantation, the mice were sacrificed, and the homing of the mMSCs was ...Continue Reading

References

Aug 12, 1967·Lancet·D G AshbaughB E Levine
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
May 22, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Boris W KramerSuhas G Kallapur
Sep 23, 2009·The Journal of Clinical Investigation·Franco R D'AlessioLandon S King
Mar 31, 2010·Cancer Immunology, Immunotherapy : CII·Yuqiang Ji, Wanggang Zhang
Sep 2, 2011·Stem Cell Reviews and Reports·Ênio José BassiNiels Olsen Saraiva Câmara
Sep 25, 2012·American Journal of Respiratory Cell and Molecular Biology·Brian T GaribaldiLandon S King
Feb 23, 2013·Respiration; International Review of Thoracic Diseases·Nayra CárdenesMauricio Rojas
Feb 17, 2015·European Journal of Immunology·WanJun Chen, Joanne E Konkel
Apr 19, 2015·Critical Care : the Official Journal of the Critical Care Forum·Zhi-xin YuJin-Xu Zheng
May 1, 2015·Drug Design, Development and Therapy·Ignacio Martin-LoechesAntonio Artigas
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Giacomo BellaniUNKNOWN ESICM Trials Group
Aug 10, 2017·The New England Journal of Medicine·B Taylor ThompsonKathleen D Liu
Dec 7, 2018·American Journal of Respiratory and Critical Care Medicine·Diana IslamHaibo Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence
flow cytometry
fluorescence-activated cell sorting
PCR
enzyme-linked immunosorbent assay
bronchoalveolar lavage
ELISA
fluorescence microscopy
light
bronchoalveolar

Software Mentioned

Image J
GraphPad Prism
SPSS
Maestro

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.