Abstract
T helper 17 cells (Th17)/regulatory T cells (Treg), as subtypes of CD4+ T cells, play an important role in the inflammatory response of acute respiratory distress syndrome (ARDS). However, there is still a lack of effective methods to regulate the differentiation balance of Th17/Treg. It was proven that mesenchymal stem cells (MSCs) could regulate the differentiation of CD4+ T cells, but the mechanism is still unclear. TGFβ1, a paracrine cytokine of MSCs, could also regulate the differentiation of Th17/Treg but is lowly expressed in MSCs. Therefore, mouse MSCs (mMSCs) overexpressing TGFβ1 were constructed by lentivirus transduction and intratracheally transplanted into LPS-induced ARDS mice in our study. The aim of this study was to evaluate the therapeutic effects of mMSCs overexpressing TGFβ1 on inflammation and immunoregulation by impacting the Th17/Treg balance in LPS-induced ARDS mice. mMSCs overexpressing TGFβ1 were constructed using lentiviral vectors. Then, mouse bone-marrow-derived MSCs (mBM-MSC) and mBM-MSC-TGFβ1 (mBM-MSC overexpressing TGFβ1) were transplanted intratracheally into ARDS mice induced by lipopolysaccharide. At 3 and 7 days after transplantation, the mice were sacrificed, and the homing of the mMSCs was ...Continue Reading
References
Aug 12, 1967·Lancet·D G AshbaughB E Levine
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
May 22, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Boris W KramerSuhas G Kallapur
Sep 23, 2009·The Journal of Clinical Investigation·Franco R D'AlessioLandon S King
Mar 31, 2010·Cancer Immunology, Immunotherapy : CII·Yuqiang Ji, Wanggang Zhang
Sep 2, 2011·Stem Cell Reviews and Reports·Ênio José BassiNiels Olsen Saraiva Câmara
Sep 25, 2012·American Journal of Respiratory Cell and Molecular Biology·Brian T GaribaldiLandon S King
Feb 23, 2013·Respiration; International Review of Thoracic Diseases·Nayra CárdenesMauricio Rojas
Jun 6, 2013·Stem Cell Research & Therapy·Patricia Luz-CrawfordFlavio Carrión
Oct 8, 2014·Cell Transplantation·Hongli HeHaibo Qiu
Feb 17, 2015·European Journal of Immunology·WanJun Chen, Joanne E Konkel
Apr 19, 2015·Critical Care : the Official Journal of the Critical Care Forum·Zhi-xin YuJin-Xu Zheng
Apr 19, 2015·Stem Cell Research & Therapy·Shi-xia CaiHai-bo Qiu
May 1, 2015·Drug Design, Development and Therapy·Ignacio Martin-LoechesAntonio Artigas
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Giacomo BellaniUNKNOWN ESICM Trials Group
May 14, 2016·Molecular Medicine Reports·Qian LiYin Ni
Aug 10, 2017·The New England Journal of Medicine·B Taylor ThompsonKathleen D Liu
Jul 11, 2018·Journal of Cellular Physiology·Xiwen ZhangFengmei Guo
Aug 23, 2018·Stem Cells Translational Medicine·Xiu-Ping XuHai-Bo Qiu
Dec 7, 2018·American Journal of Respiratory and Critical Care Medicine·Diana IslamHaibo Zhang
Jan 9, 2019·Frontiers in Immunology·Joanne Lomas-NeiraAlfred Ayala
Mar 1, 2019·Journal of Translational Medicine·Ming XueHaibo Qiu
Mar 8, 2019·Stem Cell Research & Therapy·Xiwen ZhangFengmei Guo
Mar 29, 2019·Immunology·Wen TanQi Miao
Sep 20, 2019·Nature Communications·Carla ClaserLaurent Renia
Dec 24, 2019·Cell·Huijuan WuNan Tang
Citations
Mar 20, 2021·Cell Proliferation·Yanping XuHongcui Cao
Mar 31, 2021·Life Sciences·Yueying LiShusen Cui
May 12, 2021·Journal of Translational Medicine·Wendi WangZhen-Ao Zhao
Jul 13, 2021·World Journal of Stem Cells·Amit SharmaBithiah Grace Jaganathan
Aug 23, 2021·BMC Infectious Diseases·Weina Lu, Ran Ji
Aug 31, 2021·The Kaohsiung Journal of Medical Sciences·Jing-Guo MaLin Zha