Overexpression of the alfalfa WRKY11 gene enhances salt tolerance in soybean

PloS One
Youjing WangChanghong Guo

Abstract

The WRKY transcription factors play an important role in the regulation of transcriptional reprogramming associated with plant abiotic stress responses. In this study, the WRKY transcription factor MsWRKY11, containing the plant-specific WRKY zinc finger DNA-binding motif, was isolated from alfalfa. The MsWRKY11 gene was detected in all plant tissues (root, stem, leaf, flower, and fruit), with high expression in root and leaf tissues. MsWRKY11 was upregulated in response to a variety of abiotic stresses, including salinity, alkalinity, cold, abscisic acid, and drought. Overexpression of MsWRKY11 in soybean enhanced the salt tolerance at the seedling stage. Transgenic soybean had a better salt-tolerant phenotype, and the hypocotyls were significantly longer than those of wild-type seeds after salt treatment. Furthermore, MsWRKY11 overexpression increased the contents of chlorophyll, proline, soluble sugar, superoxide dismutase, and catalase, but reduced the relative electrical conductivity and the contents of malonaldehyde, H2O2, and O2-. Plant height, pods per plant, seeds per plant, and 100-seed weight of transgenic MsWRKY11 soybean were higher than those of wild-type soybean, especially OX2. Results of the salt experiment sho...Continue Reading

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Citations

Feb 20, 2019·International Journal of Molecular Sciences·Muhammad NadeemChuanxi Ma
Dec 20, 2018·International Journal of Molecular Sciences·Wen-Yan ShiXiao-Hong Zhang
Jul 18, 2018·International Journal of Molecular Sciences·Ling HeLei Zhang
Jun 22, 2021·Frontiers in Plant Science·Shumei FangXilong Liang
Aug 28, 2021·International Journal of Molecular Sciences·Xuan Lan Thi HoangNguyen Phuong Thao

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Methods Mentioned

BETA
transgenic
MDA
salt stress
PCR
reverse transcription PCR
reverse transcription-PCR

Software Mentioned

DNAMAN
ORF Finder
Clustalx
SPSS
Primer
BLAST
Excel
SAS9

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