Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product

Biochemical and Biophysical Research Communications
Marco VanoniLilia Alberghina

Abstract

The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction.

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Citations

Nov 1, 2011·FEBS Letters·Fiorella BelottiEnzo Martegani
May 12, 2012·Biochimica Et Biophysica Acta·Fiorella BelottiEnzo Martegani
Oct 22, 2008·Biochimica Et Biophysica Acta·Renata TisiEnzo Martegani
Aug 25, 1997·Experimental Cell Research·E SturaniS Denis-Donini

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