Overexpression of the Rybp Gene Inhibits Differentiation of Bovine Myoblasts into Myotubes

International Journal of Molecular Sciences
Xiaotong SuHongbao Wang

Abstract

RING1 and YY1 binding protein (Rybp) genes inhibit myogenesis in mice, but there are no reports on the effects of these genes in cattle. The aim of this study is to investigate the roles of the Rybp gene on bovine skeletal muscle development and myoblast differentiation. In the present study, the Rybp gene was overexpressed in bovine myoblasts via adenovirus. RNA-seq was performed to screen differentially expressed genes (DEGs). The results showed that overexpressing the Rybp gene inhibits the formation of myotubes. The morphological differences in myoblasts began on the second day and were very significant 6 days after adenovirus induction. A total of 1311 (707 upregulated and 604 downregulated) DEGs were screened using RNA-seq between myoblasts with added negative control adenoviruses (AD-NC) and Rybp adenoviruses (AD-Rybp) after 6 days of induction. Gene ontology (GO) and KEGG analysis revealed that the downregulated DEGs were mainly involved in biological functions related to muscle, and, of the 32 pathways, those associated with muscle development were significantly enriched for the identified DEGs. This study can not only provide a theoretical basis for the regulation of skeletal muscle development in cattle by exploring ...Continue Reading

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Citations

Oct 22, 2020·Animals : an Open Access Journal From MDPI·Yongliang FanZhangping Yang

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Methods Mentioned

BETA
RNA-seq
PCR
light microscopy
electrophoresis

Software Mentioned

NormFinder
Bestkeeper
Java TreeView
GeNorm
KegArray

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