Overexpression of the Saccharomyces cerevisiae RER2 gene in Trichoderma reesei affects dolichol dependent enzymes and protein glycosylation

Fungal Genetics and Biology : FG & B
Urszula Perlińska-LenartJoanna Kruszewska

Abstract

Protein secretion in Trichoderma reesei could be stimulated by overexpression of the yeast Saccharomyces cerevisiae DPM1 gene encoding dolichyl phosphate mannose synthase (DPMS) a key enzyme in the O-glycosylation pathway. The secreted proteins were glycosylated to the wild type level. On the other hand, the elevated concentration of GDP-mannose, a direct substrate for DPMS, resulting from overexpression in T. reesei of the mpg1 gene coding for guanyltransferase, did not affect secretion of proteins but did affect the degree of their O- and N-glycosylation. In this paper, we examined the effects of dolichol, an indispensable carrier of sugar residues in protein glycosylation, on the synthesis of glycosylated proteins. An increase in dolichol synthesis was obtained by overexpression of the yeast gene encoding cis-prenyltransferase, the first enzyme of the mevalonate pathway committed to dolichol biosynthesis. We observed that, an increased concentration of dolichol resulted in an increased expression of the dpm1 gene and DPMS activity and in overglycosylation of secreted proteins.

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Mar 11, 2006·Fungal Genetics and Biology : FG & B·Urszula Perlińska-LenartJoanna S Kruszewska

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Citations

May 16, 2007·Molecular Microbiology·Pilar D CanteroJoachim F Ernst
Mar 11, 2006·Fungal Genetics and Biology : FG & B·Urszula Perlińska-LenartJoanna S Kruszewska
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Jul 13, 2016·The Journal of Biological Chemistry·Kariona A GrabińskaWilliam C Sessa

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