Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Plant, Cell & Environment
Huihui BiNikolai Borisjuk

Abstract

Transcription factors regulate multiple networks, mediating the responses of organisms to stresses, including drought. Here, we investigated the role of the wheat transcription factor TaSHN1 in crop growth and drought tolerance. TaSHN1, isolated from bread wheat, was characterized for molecular interactions and functionality. The overexpression of TaSHN1 in wheat was followed by the evaluation of T2 and T3 transgenic lines for drought tolerance, growth, and yield components. Leaf surface changes were analysed by light microscopy, SEM, TEM, and GC-MS/GC-FID. TaSHN1 behaves as a transcriptional activator in a yeast transactivation assay and binds stress-related DNA cis-elements, determinants of which were revealed using 3D molecular modelling. The overexpression of TaSHN1 in transgenic wheat did not result in a yield penalty under the controlled plant growth conditions of a glasshouse. Transgenic lines had significantly lower stomatal density and leaf water loss and exhibited improved recovery after severe drought, compared with control plants. The comparative analysis of cuticular waxes revealed an increased accumulation of alkanes in leaves of transgenic lines. Our data demonstrate that TaSHN1 may operate as a positive modulato...Continue Reading

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Citations

Apr 9, 2019·BioMed Research International·Nikolai BorisjukYuri Shavrukov
Feb 11, 2020·Frontiers in Plant Science·Damiano MartignagoAna I Caño-Delgado
Jul 11, 2019·International Journal of Molecular Sciences·Shahbaz KhanZhi-Qiang Gao
Oct 14, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Demissew SertseSylvie Cloutier
May 8, 2021·Plant Physiology and Biochemistry : PPB·Rania Djemal, Habib Khoudi
Jun 3, 2021·International Journal of Molecular Sciences·Maria Hrmova, Syed Sarfraz Hussain
Jun 17, 2021·Molecular Biotechnology·Olha LakhnekoBogdan Morgun

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