PMID: 11901211Mar 20, 2002Paper

Overexpression of the ubiquitin-conjugating enzyme Cdc34 confers resistance to methylmercury in Saccharomyces cerevisiae

Molecular Pharmacology
Takemitsu FuruchiAkira Naganuma

Abstract

A search was made for genes that confer resistance to methylmercury in yeast using a genomic DNA library derived from Saccharomyces cerevisiae. The genomic library was introduced into yeast and transformants that grew in the presence of a normally toxic concentration of methylmercury were selected. We sequenced the genomic DNA fragment in the plasmid from the clone with the highest resistance to methylmercury and analyzed the sequence for presence of an open reading frame that might confer resistance to methylmercury. We identified a gene, CDC34 (also known as UBC3), that increased resistance to methylmercury when overexpressed in yeast. CDC34 encodes a ubiquitin-conjugating enzyme; such proteins play important roles in the selective targeting of proteins for degradation. Overexpression of UBC4 and of UBC7, two other genes for ubiquitin-conjugating enzymes, also conferred resistance to methylmercury. Yeast strains transformed with the CDC34 gene were resistant not only to methylmercury but also to mercuric chloride and p-chloromercuribenzoate. To our knowledge, this is the first demonstration that overexpression of genes for ubiquitin-conjugating enzymes confers resistance to xenobiotics. Our results suggest that ubiquitination...Continue Reading

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Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·Takemitsu FuruchiAkira Naganuma
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Malgorzata KorbasGraham N George
Mar 3, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Gi-Wook Hwang
Sep 25, 2004·Journal of Cellular Physiology·Tsutomu TakahashiAkira Naganuma
Oct 2, 2007·Biochemical and Biophysical Research Communications·Gi-Wook HwangAkira Naganuma
Aug 5, 2009·The Journal of Toxicological Sciences·Gi-Wook HwangAkira Naganuma
Aug 5, 2009·The Journal of Toxicological Sciences·Gi-Wook HwangAkira Naganuma
Dec 25, 2002·The Tohoku Journal of Experimental Medicine·Akira NaganumaShusuke Kuge
Aug 6, 2010·The Journal of Toxicological Sciences·Gi-Wook HwangAkira Naganuma
Mar 6, 2007·Critical Reviews in Oncology/hematology·David J Stewart
Jul 19, 2002·Yeast
Sep 9, 2006·The Journal of Toxicological Sciences·Akiko KimuraAkira Naganuma
Jan 12, 2020·Biological Trace Element Research·José Cavalcante Souza VieiraPedro de Magalhães Padilha
Jul 19, 2020·Environmental Health and Preventive Medicine·Jin-Yong LeeMasahiko Satoh

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