Abstract
uPAR, the three-domain membrane receptor of the serine protease urokinase, plays a crucial role in tumor growth and metastasis. Several mRNA splice variants of this receptor have been reported. One of these, uPAR-del4/5, lacking exons 4 and 5, and thus encoding a uPAR form lacking domain DII, is specifically overexpressed in breast cancer and represents a statistically independent prognostic factor for distant metastasis-free survival in breast cancer patients. The aim of the present study was to examine the molecular and cellular properties of the encoded uPAR-del4/5 protein. To investigate the impact of the uPAR-del4/5 overexpression on in vitro and in vivo aspects of tumor progression (e.g., proliferation, adhesion, invasion, metastatic seeding, and/or metastatic growth), we combined the analysis of transfected cancer cell lines with a murine xenograft tumor model. Increased expression of uPAR-del4/5 in human cancer cells led to reduced adhesion to several extracellular matrix proteins and decreased invasion through Matrigel, while cell proliferation was not affected in vitro. Moreover, invasion of uPAR-del4/5 overexpressing cells was not altered by addition of urokinase, while that of uPAR-wild-type overexpressing cells was...Continue Reading
References
Aug 19, 1992·International Journal of Cancer. Journal International Du Cancer·V MöbusR Kreienberg
Jul 12, 1993·FEBS Letters·C PykeK Danø
Sep 13, 1996·Science·Y WeiH A Chapman
Jun 3, 1999·Cancer Metastasis Reviews·A KrügerR Khokha
Apr 19, 2000·Cancer Gene Therapy·A KrügerM Schmitt
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·Francesco Blasi, Peter Carmeliet
Jan 2, 2003·Nature Reviews. Cancer·Patricia S Steeg
Jul 23, 2003·Current Pharmaceutical Design·Michael Ploug
Sep 17, 2003·Biological Chemistry·Ute ReuningManfred Schmitt
Feb 26, 2004·Thrombosis and Haemostasis·Michelle K V DurandPeter A Andreasen
Feb 26, 2004·Thrombosis and Haemostasis·Nadia HarbeckManfred Schmitt
Feb 16, 2005·Thrombosis and Haemostasis·Daniela AlfanoMaria Patrizia Stoppelli
Apr 21, 2005·Thrombosis and Haemostasis·Buddy Setyono-HanJohn A Foekens
Apr 30, 2005·The EMBO Journal·Paola LlinasAndré Ménez
Oct 6, 2005·Cancer Research·Charlotte KopitzAchim Krüger
Nov 1, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Matthias KotzschPaul N Span
Feb 4, 2006·Science·Qing HuaiMingdong Huang
Oct 24, 2006·Gene·Thóra K BjarnadóttirHelgi B Schiöth
Jan 24, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nathalie BeaufortDominique Pidard
Mar 16, 2007·The Journal of Biological Chemistry·Henrik Gårdsvoll, Michael Ploug
Oct 24, 2007·Breast Cancer Research and Treatment·Matthias KotzschJohn A Foekens
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Apr 1, 2008·Nature Structural & Molecular Biology·Qing HuaiMingdong Huang
Dec 3, 2008·Disease Models & Mechanisms·James D Fackenthal, Lucy A Godley
Jul 30, 2009·BMC Molecular Biology·Ceri E Stewart, Ian Sayers
Nov 18, 2009·The Journal of Pathology·Amanda J Ward, Thomas A Cooper
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Harvey W Smith, Chris J Marshall
Dec 29, 2009·FEBS Letters·Francesco Blasi, Nicolai Sidenius
Citations
Feb 15, 2013·Lung·Ceri E Stewart, Ian Sayers
Jun 28, 2011·BMC Cancer·Matthias KotzschHelge Taubert
Aug 28, 2012·Molecular Cancer·Bettina GrismayerViktor Magdolen
Apr 13, 2013·PloS One·Anna LehnerEva Gross
Aug 19, 2011·Biological Chemistry·Matthias KotzschViktor Magdolen
Dec 10, 2015·Disease Markers·A R RamaJ Prados
Dec 5, 2014·Journal of Cellular and Molecular Medicine·Christelle En Lin Chua, Bor Luen Tang
May 3, 2014·Journal of the American Society of Nephrology : JASN·Dominique CathelinLaurent Mesnard
Mar 8, 2014·Molecular Medicine Reports·Yingqun ZhouLingling Zhan
Jul 3, 2018·Oncotarget·Anna Li SantiPia Ragno
Sep 2, 2014·Molecular Medicine Reports·Ying-Qun ZhouLing-Ling Zhan