Overexpression of the urokinase receptor mRNA splice variant uPAR-del4/5 affects tumor-associated processes of breast cancer cells in vitro and in vivo

Breast Cancer Research and Treatment
Sumito SatoViktor Magdolen

Abstract

uPAR, the three-domain membrane receptor of the serine protease urokinase, plays a crucial role in tumor growth and metastasis. Several mRNA splice variants of this receptor have been reported. One of these, uPAR-del4/5, lacking exons 4 and 5, and thus encoding a uPAR form lacking domain DII, is specifically overexpressed in breast cancer and represents a statistically independent prognostic factor for distant metastasis-free survival in breast cancer patients. The aim of the present study was to examine the molecular and cellular properties of the encoded uPAR-del4/5 protein. To investigate the impact of the uPAR-del4/5 overexpression on in vitro and in vivo aspects of tumor progression (e.g., proliferation, adhesion, invasion, metastatic seeding, and/or metastatic growth), we combined the analysis of transfected cancer cell lines with a murine xenograft tumor model. Increased expression of uPAR-del4/5 in human cancer cells led to reduced adhesion to several extracellular matrix proteins and decreased invasion through Matrigel, while cell proliferation was not affected in vitro. Moreover, invasion of uPAR-del4/5 overexpressing cells was not altered by addition of urokinase, while that of uPAR-wild-type overexpressing cells was...Continue Reading

References

Aug 19, 1992·International Journal of Cancer. Journal International Du Cancer·V MöbusR Kreienberg
Sep 13, 1996·Science·Y WeiH A Chapman
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·Francesco Blasi, Peter Carmeliet
Jan 2, 2003·Nature Reviews. Cancer·Patricia S Steeg
Feb 26, 2004·Thrombosis and Haemostasis·Michelle K V DurandPeter A Andreasen
Feb 16, 2005·Thrombosis and Haemostasis·Daniela AlfanoMaria Patrizia Stoppelli
Nov 1, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Matthias KotzschPaul N Span
Oct 24, 2006·Gene·Thóra K BjarnadóttirHelgi B Schiöth
Jan 24, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nathalie BeaufortDominique Pidard
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Apr 1, 2008·Nature Structural & Molecular Biology·Qing HuaiMingdong Huang
Dec 3, 2008·Disease Models & Mechanisms·James D Fackenthal, Lucy A Godley
Nov 18, 2009·The Journal of Pathology·Amanda J Ward, Thomas A Cooper
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Harvey W Smith, Chris J Marshall
Dec 29, 2009·FEBS Letters·Francesco Blasi, Nicolai Sidenius

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Citations

Aug 28, 2012·Molecular Cancer·Bettina GrismayerViktor Magdolen
Dec 10, 2015·Disease Markers·A R RamaJ Prados
Dec 5, 2014·Journal of Cellular and Molecular Medicine·Christelle En Lin Chua, Bor Luen Tang
May 3, 2014·Journal of the American Society of Nephrology : JASN·Dominique CathelinLaurent Mesnard

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