Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes

Cytotechnology
Yi DingCunshuan Xu

Abstract

Hepatocytes differentiated from induced pluripotent stem cells and adult stem cells could be utilized as a tool for the study of liver diseases, screening for drug metabolism and hepatotoxicity. Thus further investigation of the method to efficiently generate hepatocytes is in great need. Bone Mesenchymal Stem Cells (BMSCs) were collected from rat femurs and tibias. FOXA2 and HNF1α genes were constructed into a lentiviral vector and introduced into BMSCs by a lentivirus-mediated overexpression system. Three weeks after the induction, the expressions of FOXA2 and HNF1α, and liver specific genes were analyzed, and hepatocyte-function related assays were performed. Overexpression of both FOXA2 and HNF1α induced the BMSCs to differentiate into hepatocyte-like cells (HLCs). Hepatocyte-specific gene and protein were detected by RT-PCR, Western Blot and Immunofluorescence. These HLCs also exerted some typical hepatocyte functions such as glycogen storage, indocyanine green absorption and lipid accumulation. The combination of FOXA2 and HNF1α can effectively induce BMSCs to differentiate into HLCs. This is a novel and efficient method to prepare HLCs within a short timeline.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1987·Cell and Tissue Kinetics·A J FriedensteinU V Gerasimov
Sep 1, 1968·Annals of Surgery·T E StarzlK A Porter
Jun 9, 2001·Experimental Biology and Medicine·J J MinguellP Conget
Jun 14, 2002·Biochemical and Biophysical Research Communications·Rainer KlockeDieter Paul
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Agnieszka BanasTakahiro Ochiya
Jul 11, 2007·Journal of Cellular Physiology·Arnold I Caplan
Feb 12, 2008·Current Protocols in Molecular Biology·Robert E KingstonJohn K Rose
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
May 14, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kristen J SkvorakStephen C Strom
Oct 14, 2009·Journal of Gastroenterology and Hepatology·M Thamara P R PereraElwyn Elias
Nov 21, 2009·Cytokine & Growth Factor Reviews·Lindolfo da Silva MeirellesArnold I Caplan
Dec 2, 2009·Journal of Cellular Biochemistry·Masanobu Ohishi, Ernestina Schipani
Mar 18, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Thomas E Starzl, John J Fung
May 29, 2010·Neurology India·Naresh PolisettiGeeta K Vemuganti
Jul 9, 2010·World Journal of Gastroenterology : WJG·Xue-Jun DongJian-Zhong Shao
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Stevan A Gonzalez, Emmet B Keeffe
Aug 6, 2011·Cell Stem Cell·Holger Willenbring
Nov 10, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuo TakayamaHiroyuki Mizuguchi

❮ Previous
Next ❯

Citations

Jan 19, 2018·PloS One·Hye Jin ChangJae-Wook Jeong
Nov 7, 2019·Journal of Cellular and Molecular Medicine·Chenxia HuLanjuan Li
Sep 22, 2017·Cellular & Molecular Biology Letters·Chunyan ZhangCunshuan Xu
Jan 4, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Chenxia HuLanjuan Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.