Overexpression of Transglutaminase from Cucumber in Tobacco Increases Salt Tolerance through Regulation of Photosynthesis

International Journal of Molecular Sciences
Min ZhongShi-Rong Guo

Abstract

Transglutaminase (TGase) is a regulator of posttranslational modification of protein that provides physiological protection against diverse environmental stresses in plants. Nonetheless, the mechanisms of TGase-mediated salt tolerance remain largely unknown. Here, we found that the transcription of cucumber TGase (CsTGase) was induced in response to light and during leaf development, and the CsTGase protein was expressed in the chloroplast and the cell wall. The overexpression of the CsTGase gene effectively ameliorated salt-induced photoinhibition in tobacco plants, increased the levels of chloroplast polyamines (PAs) and enhanced the abundance of D1 and D2 proteins. TGase also induced the expression of photosynthesis related genes and remodeling of thylakoids under normal conditions. However, salt stress treatment reduced the photosynthesis rate, PSII and PSI related genes expression, D1 and D2 proteins in wild-type (WT) plants, while these effects were alleviated in CsTGase overexpression plants. Taken together, our results indicate that TGase-dependent PA signaling protects the proteins of thylakoids, which plays a critical role in plant response to salt stress. Thus, overexpression of TGase may be an effective strategy for...Continue Reading

References

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Citations

May 18, 2019·International Journal of Molecular Sciences·Jose Antonio Hernández
Oct 16, 2020·International Journal of Molecular Sciences·Haruhiko JimboHajime Wada
May 27, 2021·Plant Science : an International Journal of Experimental Plant Biology·Magda PálTibor Janda

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Datasets Mentioned

BETA
AS01004

Methods Mentioned

BETA
environmental stresses
salt stress
PCR
Transgenic
transmission electron microscopy
Fluorescence
Protein Assay

Software Mentioned

Quantity One
PAM
SPSS
Dual

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